Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients

To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMate(TM) isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cel...

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Autores principales: Efthymiou, Athina, Mureanu, Nicoleta, Pemberton, Rebecca, Tai-MacArthur, Sarah, Mastronicola, Daniela, Scottà, Cristiano, Lombardi, Giovanna, Nicolaides, Kypros H., Shangaris, Panicos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888982/
https://www.ncbi.nlm.nih.gov/pubmed/35252886
http://dx.doi.org/10.1016/j.xpro.2022.101204
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author Efthymiou, Athina
Mureanu, Nicoleta
Pemberton, Rebecca
Tai-MacArthur, Sarah
Mastronicola, Daniela
Scottà, Cristiano
Lombardi, Giovanna
Nicolaides, Kypros H.
Shangaris, Panicos
author_facet Efthymiou, Athina
Mureanu, Nicoleta
Pemberton, Rebecca
Tai-MacArthur, Sarah
Mastronicola, Daniela
Scottà, Cristiano
Lombardi, Giovanna
Nicolaides, Kypros H.
Shangaris, Panicos
author_sort Efthymiou, Athina
collection PubMed
description To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMate(TM) isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html.
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spelling pubmed-88889822022-03-03 Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients Efthymiou, Athina Mureanu, Nicoleta Pemberton, Rebecca Tai-MacArthur, Sarah Mastronicola, Daniela Scottà, Cristiano Lombardi, Giovanna Nicolaides, Kypros H. Shangaris, Panicos STAR Protoc Protocol To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMate(TM) isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html. Elsevier 2022-02-25 /pmc/articles/PMC8888982/ /pubmed/35252886 http://dx.doi.org/10.1016/j.xpro.2022.101204 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Efthymiou, Athina
Mureanu, Nicoleta
Pemberton, Rebecca
Tai-MacArthur, Sarah
Mastronicola, Daniela
Scottà, Cristiano
Lombardi, Giovanna
Nicolaides, Kypros H.
Shangaris, Panicos
Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients
title Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients
title_full Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients
title_fullStr Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients
title_full_unstemmed Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients
title_short Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients
title_sort isolation and freezing of human peripheral blood mononuclear cells from pregnant patients
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888982/
https://www.ncbi.nlm.nih.gov/pubmed/35252886
http://dx.doi.org/10.1016/j.xpro.2022.101204
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