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Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study
BACKGROUND: In Aayurveda, Blumea eriantha DC has been used in the management of various diseases and is found to exhibit antioxidant and anti-hyperlipidemic, hypoglycemic, anti-diarrhoeal, larvicidal, antimicrobial properties. OBJECTIVE: The present study has focused on isolation of the active fract...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888983/ https://www.ncbi.nlm.nih.gov/pubmed/35228151 http://dx.doi.org/10.1016/j.jaim.2022.100542 |
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author | Bhinge, Somnath D. Jadhav, Namdeo R. Randive, Dheeraj S. Bhutkar, Mangesh A. Chavan, Rohankumar Kumbhar, Bajarang V. |
author_facet | Bhinge, Somnath D. Jadhav, Namdeo R. Randive, Dheeraj S. Bhutkar, Mangesh A. Chavan, Rohankumar Kumbhar, Bajarang V. |
author_sort | Bhinge, Somnath D. |
collection | PubMed |
description | BACKGROUND: In Aayurveda, Blumea eriantha DC has been used in the management of various diseases and is found to exhibit antioxidant and anti-hyperlipidemic, hypoglycemic, anti-diarrhoeal, larvicidal, antimicrobial properties. OBJECTIVE: The present study has focused on isolation of the active fraction from B. eriantha DC extract and to investigate its effect as a hair growth promoter along with identification of phytoconstituent(s) responsible for hair growth activity and its probable mechanism of action. MATERIALS AND METHODS: Our work introduces an effective isolation protocol for the active fraction from B. eriantha DC extract using chromatographic techniques. Fraction A was isolated by using mobile phase toluene:acetone (9:1). In-vitro and in-vivo methods were executed for the evaluation of hair growth activity. Moreover, the docked conformations of the isolated phytoconstituent Dimethyl sulfone was compared to Minoxidil for selected proteins namely 2FGF, 2PVC and 4U7P. The PDB identifications 2PVC (DNMT3L recognizes unmethylated histone H3 lysine 4), 4U7P (Crystal structure of DNMT3A-DNMT3L complex and 2FGF (Human Basic Fibroblast Growth Factor) were downloaded from Protein Data Bank. RESULTS: The study data revealed that B. eriantha DC alcoholic extracts exhibited prominent hair growth activity and it was affirmed that Dimethyl sulfone a phyto-constituent isolated from B. eriantha DC alcoholic extract contributed for the same. CONCLUSION: The findings strongly suggest hair growth promotion potential of the extract of B. eriantha DC. |
format | Online Article Text |
id | pubmed-8888983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-88889832022-03-03 Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study Bhinge, Somnath D. Jadhav, Namdeo R. Randive, Dheeraj S. Bhutkar, Mangesh A. Chavan, Rohankumar Kumbhar, Bajarang V. J Ayurveda Integr Med Original Research Article BACKGROUND: In Aayurveda, Blumea eriantha DC has been used in the management of various diseases and is found to exhibit antioxidant and anti-hyperlipidemic, hypoglycemic, anti-diarrhoeal, larvicidal, antimicrobial properties. OBJECTIVE: The present study has focused on isolation of the active fraction from B. eriantha DC extract and to investigate its effect as a hair growth promoter along with identification of phytoconstituent(s) responsible for hair growth activity and its probable mechanism of action. MATERIALS AND METHODS: Our work introduces an effective isolation protocol for the active fraction from B. eriantha DC extract using chromatographic techniques. Fraction A was isolated by using mobile phase toluene:acetone (9:1). In-vitro and in-vivo methods were executed for the evaluation of hair growth activity. Moreover, the docked conformations of the isolated phytoconstituent Dimethyl sulfone was compared to Minoxidil for selected proteins namely 2FGF, 2PVC and 4U7P. The PDB identifications 2PVC (DNMT3L recognizes unmethylated histone H3 lysine 4), 4U7P (Crystal structure of DNMT3A-DNMT3L complex and 2FGF (Human Basic Fibroblast Growth Factor) were downloaded from Protein Data Bank. RESULTS: The study data revealed that B. eriantha DC alcoholic extracts exhibited prominent hair growth activity and it was affirmed that Dimethyl sulfone a phyto-constituent isolated from B. eriantha DC alcoholic extract contributed for the same. CONCLUSION: The findings strongly suggest hair growth promotion potential of the extract of B. eriantha DC. Elsevier 2022 2022-02-25 /pmc/articles/PMC8888983/ /pubmed/35228151 http://dx.doi.org/10.1016/j.jaim.2022.100542 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Article Bhinge, Somnath D. Jadhav, Namdeo R. Randive, Dheeraj S. Bhutkar, Mangesh A. Chavan, Rohankumar Kumbhar, Bajarang V. Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study |
title | Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study |
title_full | Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study |
title_fullStr | Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study |
title_full_unstemmed | Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study |
title_short | Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study |
title_sort | isolation and identification of hair growth potential fraction from active plant extract of blumea eriantha dc grown in western ghat of india: in silico study |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888983/ https://www.ncbi.nlm.nih.gov/pubmed/35228151 http://dx.doi.org/10.1016/j.jaim.2022.100542 |
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