A toolbox to study metabolic status of Drosophila melanogaster larvae

Somatic energy reserves are essential for reproductive success and can govern the onset of sexual maturation. Here, we present a toolkit to analyze the metabolic status of Drosophila larvae using an optimized NMR profiling assay in dissected tissues or whole animals, as well as a complementary proto...

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Detalles Bibliográficos
Autores principales: Palomino-Schätzlein, Martina, Carranza-Valencia, Juan, Guirado, Juan, Juarez-Carreño, Sergio, Morante, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888985/
https://www.ncbi.nlm.nih.gov/pubmed/35252884
http://dx.doi.org/10.1016/j.xpro.2022.101195
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author Palomino-Schätzlein, Martina
Carranza-Valencia, Juan
Guirado, Juan
Juarez-Carreño, Sergio
Morante, Javier
author_facet Palomino-Schätzlein, Martina
Carranza-Valencia, Juan
Guirado, Juan
Juarez-Carreño, Sergio
Morante, Javier
author_sort Palomino-Schätzlein, Martina
collection PubMed
description Somatic energy reserves are essential for reproductive success and can govern the onset of sexual maturation. Here, we present a toolkit to analyze the metabolic status of Drosophila larvae using an optimized NMR profiling assay in dissected tissues or whole animals, as well as a complementary protocol for the dissection and staining of key organs in nutrient sensing. This toolkit will aid investigations into critical body weight signaling and how it is sensed for maturation commitment in Drosophila. For complete details on the use and execution of this profile, please refer to Juarez-Carreño et al. (2021).
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spelling pubmed-88889852022-03-03 A toolbox to study metabolic status of Drosophila melanogaster larvae Palomino-Schätzlein, Martina Carranza-Valencia, Juan Guirado, Juan Juarez-Carreño, Sergio Morante, Javier STAR Protoc Protocol Somatic energy reserves are essential for reproductive success and can govern the onset of sexual maturation. Here, we present a toolkit to analyze the metabolic status of Drosophila larvae using an optimized NMR profiling assay in dissected tissues or whole animals, as well as a complementary protocol for the dissection and staining of key organs in nutrient sensing. This toolkit will aid investigations into critical body weight signaling and how it is sensed for maturation commitment in Drosophila. For complete details on the use and execution of this profile, please refer to Juarez-Carreño et al. (2021). Elsevier 2022-02-24 /pmc/articles/PMC8888985/ /pubmed/35252884 http://dx.doi.org/10.1016/j.xpro.2022.101195 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Palomino-Schätzlein, Martina
Carranza-Valencia, Juan
Guirado, Juan
Juarez-Carreño, Sergio
Morante, Javier
A toolbox to study metabolic status of Drosophila melanogaster larvae
title A toolbox to study metabolic status of Drosophila melanogaster larvae
title_full A toolbox to study metabolic status of Drosophila melanogaster larvae
title_fullStr A toolbox to study metabolic status of Drosophila melanogaster larvae
title_full_unstemmed A toolbox to study metabolic status of Drosophila melanogaster larvae
title_short A toolbox to study metabolic status of Drosophila melanogaster larvae
title_sort toolbox to study metabolic status of drosophila melanogaster larvae
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888985/
https://www.ncbi.nlm.nih.gov/pubmed/35252884
http://dx.doi.org/10.1016/j.xpro.2022.101195
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