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Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass

Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables more economical lignocellulosic biorefinery with improved production yields per unit of biomass. Yet, the product profile of glucose/xylose co-fermenting S. cerevisiae is mainly limited to bioethanol and a few other chemicals...

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Autores principales: Lee, Yeon-Jung, Hoang Nguyen Tran, Phuong, Ko, Ja Kyong, Gong, Gyeongtaek, Um, Youngsoon, Han, Sung Ok, Lee, Sun-Mi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889018/
https://www.ncbi.nlm.nih.gov/pubmed/35252135
http://dx.doi.org/10.3389/fbioe.2022.826787
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author Lee, Yeon-Jung
Hoang Nguyen Tran, Phuong
Ko, Ja Kyong
Gong, Gyeongtaek
Um, Youngsoon
Han, Sung Ok
Lee, Sun-Mi
author_facet Lee, Yeon-Jung
Hoang Nguyen Tran, Phuong
Ko, Ja Kyong
Gong, Gyeongtaek
Um, Youngsoon
Han, Sung Ok
Lee, Sun-Mi
author_sort Lee, Yeon-Jung
collection PubMed
description Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables more economical lignocellulosic biorefinery with improved production yields per unit of biomass. Yet, the product profile of glucose/xylose co-fermenting S. cerevisiae is mainly limited to bioethanol and a few other chemicals. Here, we introduced an n-butanol-biosynthesis pathway into a glucose/xylose co-fermenting S. cerevisiae strain (XUSEA) to evaluate its potential on the production of acetyl-CoA derived products. Higher n-butanol production of glucose/xylose co-fermenting strain was explained by the transcriptomic landscape, which revealed strongly increased acetyl-CoA and NADPH pools when compared to a glucose fermenting wild-type strain. The acetate supplementation expected to support acetyl-CoA pool further increased n-butanol production, which was also validated during the fermentation of lignocellulosic hydrolysates containing acetate. Our findings imply the feasibility of lignocellulosic biorefinery for producing fuels and chemicals derived from a key intermediate of acetyl-CoA through glucose/xylose co-fermentation.
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spelling pubmed-88890182022-03-03 Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass Lee, Yeon-Jung Hoang Nguyen Tran, Phuong Ko, Ja Kyong Gong, Gyeongtaek Um, Youngsoon Han, Sung Ok Lee, Sun-Mi Front Bioeng Biotechnol Bioengineering and Biotechnology Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables more economical lignocellulosic biorefinery with improved production yields per unit of biomass. Yet, the product profile of glucose/xylose co-fermenting S. cerevisiae is mainly limited to bioethanol and a few other chemicals. Here, we introduced an n-butanol-biosynthesis pathway into a glucose/xylose co-fermenting S. cerevisiae strain (XUSEA) to evaluate its potential on the production of acetyl-CoA derived products. Higher n-butanol production of glucose/xylose co-fermenting strain was explained by the transcriptomic landscape, which revealed strongly increased acetyl-CoA and NADPH pools when compared to a glucose fermenting wild-type strain. The acetate supplementation expected to support acetyl-CoA pool further increased n-butanol production, which was also validated during the fermentation of lignocellulosic hydrolysates containing acetate. Our findings imply the feasibility of lignocellulosic biorefinery for producing fuels and chemicals derived from a key intermediate of acetyl-CoA through glucose/xylose co-fermentation. Frontiers Media S.A. 2022-02-16 /pmc/articles/PMC8889018/ /pubmed/35252135 http://dx.doi.org/10.3389/fbioe.2022.826787 Text en Copyright © 2022 Lee, Hoang Nguyen Tran, Ko, Gong, Um, Han and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Lee, Yeon-Jung
Hoang Nguyen Tran, Phuong
Ko, Ja Kyong
Gong, Gyeongtaek
Um, Youngsoon
Han, Sung Ok
Lee, Sun-Mi
Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass
title Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass
title_full Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass
title_fullStr Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass
title_full_unstemmed Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass
title_short Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass
title_sort glucose/xylose co-fermenting saccharomyces cerevisiae increases the production of acetyl-coa derived n-butanol from lignocellulosic biomass
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889018/
https://www.ncbi.nlm.nih.gov/pubmed/35252135
http://dx.doi.org/10.3389/fbioe.2022.826787
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