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Simultaneous TLC-densitometric analysis of catechin, pyrocatechol and quercetine in gambir block from Pesisir Selatan
The goal of this study is to use the TLC-densitometric method to determine the concentration of catechin, pyrocatechol, and quercetine in gambir block extracts in a reliable and efficient manner. The best eluent is a mixture of chloroform: ethyl acetate: glacial acetic acid (4:4:2). The concentratio...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889343/ https://www.ncbi.nlm.nih.gov/pubmed/35252606 http://dx.doi.org/10.1016/j.heliyon.2022.e08985 |
| Sumario: | The goal of this study is to use the TLC-densitometric method to determine the concentration of catechin, pyrocatechol, and quercetine in gambir block extracts in a reliable and efficient manner. The best eluent is a mixture of chloroform: ethyl acetate: glacial acetic acid (4:4:2). The concentration of catechin, pyrocatechol and quercetine in gambir block was found to be 25.50 ± 3.13, 0.91 ± 0.60 and 0.83 ± 0.34% (w/w) w/w respectively. The linearity was obtained between 750-2500, 50–350, and 50–350 μg/spot. 12.49–41.63, 0.48–1.60, 3,85–12.83 μg/spots were found to represent the LOD and LOQ, respectively. The proposed approach exhibited great sensitivity, precision, and accuracy, as well as strong linearity. |
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