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Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold

[Image: see text] DNA nanotechnology has increasingly been used as a platform to scaffold enzymes based on its unmatched ability to structure enzymes in a desired format. The capability to organize enzymes has taken many forms from more traditional 2D pairings on individual scaffolds to recent works...

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Autores principales: Kahn, Jason S., Xiong, Yan, Huang, James, Gang, Oleg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889550/
https://www.ncbi.nlm.nih.gov/pubmed/35252986
http://dx.doi.org/10.1021/jacsau.1c00387
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author Kahn, Jason S.
Xiong, Yan
Huang, James
Gang, Oleg
author_facet Kahn, Jason S.
Xiong, Yan
Huang, James
Gang, Oleg
author_sort Kahn, Jason S.
collection PubMed
description [Image: see text] DNA nanotechnology has increasingly been used as a platform to scaffold enzymes based on its unmatched ability to structure enzymes in a desired format. The capability to organize enzymes has taken many forms from more traditional 2D pairings on individual scaffolds to recent works introducing enzyme organizations in 3D lattices. As the ability to define nanoscale structure has grown, it is critical to fully deconstruct the impact of enzyme organization at the single-scaffold level. Here, we present an open, three-dimensional (3D) DNA wireframe octahedron which is used to create a library of spatially arranged organizations of glucose oxidase and horseradish peroxidase. We explore the contribution of enzyme spacing, arrangement, and location on the 3D scaffold to cascade activity. The experiments provide insight into enzyme scaffold design, including the insignificance of scaffold sequence makeup on activity, an increase in activity at small enzyme spacings of <10 nm, and activity changes that arise from discontinuities in scaffold architecture. Most notably, the experiments allow us to determine that enzyme colocalization itself on the DNA scaffold dominates over any specific enzyme arrangement.
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spelling pubmed-88895502022-03-03 Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold Kahn, Jason S. Xiong, Yan Huang, James Gang, Oleg JACS Au [Image: see text] DNA nanotechnology has increasingly been used as a platform to scaffold enzymes based on its unmatched ability to structure enzymes in a desired format. The capability to organize enzymes has taken many forms from more traditional 2D pairings on individual scaffolds to recent works introducing enzyme organizations in 3D lattices. As the ability to define nanoscale structure has grown, it is critical to fully deconstruct the impact of enzyme organization at the single-scaffold level. Here, we present an open, three-dimensional (3D) DNA wireframe octahedron which is used to create a library of spatially arranged organizations of glucose oxidase and horseradish peroxidase. We explore the contribution of enzyme spacing, arrangement, and location on the 3D scaffold to cascade activity. The experiments provide insight into enzyme scaffold design, including the insignificance of scaffold sequence makeup on activity, an increase in activity at small enzyme spacings of <10 nm, and activity changes that arise from discontinuities in scaffold architecture. Most notably, the experiments allow us to determine that enzyme colocalization itself on the DNA scaffold dominates over any specific enzyme arrangement. American Chemical Society 2022-01-07 /pmc/articles/PMC8889550/ /pubmed/35252986 http://dx.doi.org/10.1021/jacsau.1c00387 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Kahn, Jason S.
Xiong, Yan
Huang, James
Gang, Oleg
Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold
title Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold
title_full Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold
title_fullStr Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold
title_full_unstemmed Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold
title_short Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold
title_sort cascaded enzyme reactions over a three-dimensional, wireframe dna origami scaffold
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889550/
https://www.ncbi.nlm.nih.gov/pubmed/35252986
http://dx.doi.org/10.1021/jacsau.1c00387
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