DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electropho...

Descripción completa

Detalles Bibliográficos
Autores principales: Martínez-Roque, Mateo Alejandro, Franco-Urquijo, Pablo Alberto, García-Velásquez, Víctor Miguel, Choukeife, Moujab, Mayer, Günther, Molina-Ramírez, Sergio Roberto, Figueroa-Miranda, Gabriela, Mayer, Dirk, Alvarez-Salas, Luis M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889740/
https://www.ncbi.nlm.nih.gov/pubmed/35247355
http://dx.doi.org/10.1016/j.ab.2022.114633
Descripción
Sumario:The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively.

Ejemplares similares