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Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans

The ascomycete fungus Cordyceps militaris infects lepidopteran larvae and pupae and forms characteristic fruiting bodies. Owing to its immune-enhancing effects, the fungus has been used as a medicine. For industrial application, this fungus can be grown on geminated soybeans as an alternative protei...

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Autores principales: Yoo, Chang-Hyuk, Sadat, Md. Abu, Kim, Wonjae, Park, Tae-Sik, Park, Dong Ki, Choi, Jaehyuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8890544/
https://www.ncbi.nlm.nih.gov/pubmed/35291592
http://dx.doi.org/10.1080/12298093.2022.2035906
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author Yoo, Chang-Hyuk
Sadat, Md. Abu
Kim, Wonjae
Park, Tae-Sik
Park, Dong Ki
Choi, Jaehyuk
author_facet Yoo, Chang-Hyuk
Sadat, Md. Abu
Kim, Wonjae
Park, Tae-Sik
Park, Dong Ki
Choi, Jaehyuk
author_sort Yoo, Chang-Hyuk
collection PubMed
description The ascomycete fungus Cordyceps militaris infects lepidopteran larvae and pupae and forms characteristic fruiting bodies. Owing to its immune-enhancing effects, the fungus has been used as a medicine. For industrial application, this fungus can be grown on geminated soybeans as an alternative protein source. In our study, we performed a comprehensive transcriptomic analysis to identify core gene sets during C. militaris cultivation on germinated soybeans. RNA-Seq technology was applied to the fungal cultures at seven-time points (2, 4, and 7-day and 2, 3, 5, 7-week old cultures) to investigate the global transcriptomic change. We conducted a time-series analysis using a two-step regression strategy and chose 1460 significant genes and assigned them into five clusters. Characterization of each cluster based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that transcription profiles changed after two weeks of incubation. Gene mapping of cordycepin biosynthesis and isoflavone modification pathways also confirmed that gene expression in the early stage of GSC cultivation is important for these metabolic pathways. Our transcriptomic analysis and selected genes provided a comprehensive molecular basis for the cultivation of C. militaris on germinated soybeans.
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spelling pubmed-88905442022-03-14 Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans Yoo, Chang-Hyuk Sadat, Md. Abu Kim, Wonjae Park, Tae-Sik Park, Dong Ki Choi, Jaehyuk Mycobiology Review Article The ascomycete fungus Cordyceps militaris infects lepidopteran larvae and pupae and forms characteristic fruiting bodies. Owing to its immune-enhancing effects, the fungus has been used as a medicine. For industrial application, this fungus can be grown on geminated soybeans as an alternative protein source. In our study, we performed a comprehensive transcriptomic analysis to identify core gene sets during C. militaris cultivation on germinated soybeans. RNA-Seq technology was applied to the fungal cultures at seven-time points (2, 4, and 7-day and 2, 3, 5, 7-week old cultures) to investigate the global transcriptomic change. We conducted a time-series analysis using a two-step regression strategy and chose 1460 significant genes and assigned them into five clusters. Characterization of each cluster based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that transcription profiles changed after two weeks of incubation. Gene mapping of cordycepin biosynthesis and isoflavone modification pathways also confirmed that gene expression in the early stage of GSC cultivation is important for these metabolic pathways. Our transcriptomic analysis and selected genes provided a comprehensive molecular basis for the cultivation of C. militaris on germinated soybeans. Taylor & Francis 2022-02-24 /pmc/articles/PMC8890544/ /pubmed/35291592 http://dx.doi.org/10.1080/12298093.2022.2035906 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of the Korean Society of Mycology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Yoo, Chang-Hyuk
Sadat, Md. Abu
Kim, Wonjae
Park, Tae-Sik
Park, Dong Ki
Choi, Jaehyuk
Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
title Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
title_full Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
title_fullStr Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
title_full_unstemmed Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
title_short Comprehensive Transcriptomic Analysis of Cordyceps militaris Cultivated on Germinated Soybeans
title_sort comprehensive transcriptomic analysis of cordyceps militaris cultivated on germinated soybeans
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8890544/
https://www.ncbi.nlm.nih.gov/pubmed/35291592
http://dx.doi.org/10.1080/12298093.2022.2035906
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