Cargando…
Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells
Chlamydia trachomatis (C. trachomatis) is a major etiological agent of sexually transmitted infection. Some stressing conditions can result in persistent chlamydial infection, which is thought to be associated with severe complications including ectopic pregnancy and tubal factor infertility. Long n...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8890745/ https://www.ncbi.nlm.nih.gov/pubmed/35252346 http://dx.doi.org/10.3389/fmolb.2022.744901 |
_version_ | 1784661711202025472 |
---|---|
author | Huang, Xiaobao Liufu, Qian Xu, Rui Chen, Xiaohong Liu, Mingna Han, Jiande Guan, Hongyu Ma, Chunguang |
author_facet | Huang, Xiaobao Liufu, Qian Xu, Rui Chen, Xiaohong Liu, Mingna Han, Jiande Guan, Hongyu Ma, Chunguang |
author_sort | Huang, Xiaobao |
collection | PubMed |
description | Chlamydia trachomatis (C. trachomatis) is a major etiological agent of sexually transmitted infection. Some stressing conditions can result in persistent chlamydial infection, which is thought to be associated with severe complications including ectopic pregnancy and tubal factor infertility. Long noncoding RNAs (lncRNAs) have been identified as key modulators in many biological processes. Nevertheless, the role of lncRNAs in persistent chlamydial infection is still unclear. In this study, we used lncRNA and mRNA microarray to identify the global lncRNAs and mRNAs expression in penicillin-induced persistent chlamydial infection in HeLa cells as well as the control group (HeLa cells without C. trachomatis infection). Among 1005 differentially expressed lncRNAs, 585 lncRNAs were upregulated and 420 downregulated in persistent chlamydial infection, while 410 mRNAs were identified to express differentially, of which 113 mRNAs were upregulated and 297 downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis with differentially expressed genes were performed. We then constructed the lncRNA-miRNA-mRNA competing endogenous RNAs (ceRNAs) network. Four mRNAs were validated to be changed by quantitative real-time PCR which were correlated with the microarray result. Integration of protein-protein interaction network was constructed and hub genes were identified. These findings provide a new perspective on the molecular mechanisms of penicillin-induced persistent chlamydial infection. |
format | Online Article Text |
id | pubmed-8890745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88907452022-03-03 Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells Huang, Xiaobao Liufu, Qian Xu, Rui Chen, Xiaohong Liu, Mingna Han, Jiande Guan, Hongyu Ma, Chunguang Front Mol Biosci Molecular Biosciences Chlamydia trachomatis (C. trachomatis) is a major etiological agent of sexually transmitted infection. Some stressing conditions can result in persistent chlamydial infection, which is thought to be associated with severe complications including ectopic pregnancy and tubal factor infertility. Long noncoding RNAs (lncRNAs) have been identified as key modulators in many biological processes. Nevertheless, the role of lncRNAs in persistent chlamydial infection is still unclear. In this study, we used lncRNA and mRNA microarray to identify the global lncRNAs and mRNAs expression in penicillin-induced persistent chlamydial infection in HeLa cells as well as the control group (HeLa cells without C. trachomatis infection). Among 1005 differentially expressed lncRNAs, 585 lncRNAs were upregulated and 420 downregulated in persistent chlamydial infection, while 410 mRNAs were identified to express differentially, of which 113 mRNAs were upregulated and 297 downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis with differentially expressed genes were performed. We then constructed the lncRNA-miRNA-mRNA competing endogenous RNAs (ceRNAs) network. Four mRNAs were validated to be changed by quantitative real-time PCR which were correlated with the microarray result. Integration of protein-protein interaction network was constructed and hub genes were identified. These findings provide a new perspective on the molecular mechanisms of penicillin-induced persistent chlamydial infection. Frontiers Media S.A. 2022-02-16 /pmc/articles/PMC8890745/ /pubmed/35252346 http://dx.doi.org/10.3389/fmolb.2022.744901 Text en Copyright © 2022 Huang, Liufu, Xu, Chen, Liu, Han, Guan and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Huang, Xiaobao Liufu, Qian Xu, Rui Chen, Xiaohong Liu, Mingna Han, Jiande Guan, Hongyu Ma, Chunguang Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
title | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
title_full | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
title_fullStr | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
title_full_unstemmed | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
title_short | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
title_sort | integrating lncrnas and mrnas expression profiles in penicillin-induced persistent chlamydial infection in hela cells |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8890745/ https://www.ncbi.nlm.nih.gov/pubmed/35252346 http://dx.doi.org/10.3389/fmolb.2022.744901 |
work_keys_str_mv | AT huangxiaobao integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells AT liufuqian integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells AT xurui integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells AT chenxiaohong integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells AT liumingna integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells AT hanjiande integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells AT guanhongyu integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells AT machunguang integratinglncrnasandmrnasexpressionprofilesinpenicillininducedpersistentchlamydialinfectioninhelacells |