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Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing

The increasing mutation frequency of the SARS-CoV-2 virus and the emergence of successive variants have made correct diagnosis hard to perform. Developing efficient and accurate methods to diagnose infected patients is crucial to effectively mitigate the pandemic. Here, we developed an electrochemic...

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Autores principales: Durmus, Ceren, Balaban Hanoglu, Simge, Harmanci, Duygu, Moulahoum, Hichem, Tok, Kerem, Ghorbanizamani, Faezeh, Sanli, Serdar, Zihnioglu, Figen, Evran, Serap, Cicek, Candan, Sertoz, Ruchan, Arda, Bilgin, Goksel, Tuncay, Turhan, Kutsal, Timur, Suna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891155/
https://www.ncbi.nlm.nih.gov/pubmed/35248943
http://dx.doi.org/10.1016/j.talanta.2022.123356
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author Durmus, Ceren
Balaban Hanoglu, Simge
Harmanci, Duygu
Moulahoum, Hichem
Tok, Kerem
Ghorbanizamani, Faezeh
Sanli, Serdar
Zihnioglu, Figen
Evran, Serap
Cicek, Candan
Sertoz, Ruchan
Arda, Bilgin
Goksel, Tuncay
Turhan, Kutsal
Timur, Suna
author_facet Durmus, Ceren
Balaban Hanoglu, Simge
Harmanci, Duygu
Moulahoum, Hichem
Tok, Kerem
Ghorbanizamani, Faezeh
Sanli, Serdar
Zihnioglu, Figen
Evran, Serap
Cicek, Candan
Sertoz, Ruchan
Arda, Bilgin
Goksel, Tuncay
Turhan, Kutsal
Timur, Suna
author_sort Durmus, Ceren
collection PubMed
description The increasing mutation frequency of the SARS-CoV-2 virus and the emergence of successive variants have made correct diagnosis hard to perform. Developing efficient and accurate methods to diagnose infected patients is crucial to effectively mitigate the pandemic. Here, we developed an electrochemical immunosensor based on SARS-CoV-2 antibody cocktail-conjugated magnetic nanoparticles for the sensitive and accurate detection of the SARS-CoV-2 virus and its variants in nasopharyngeal swabs. The application of the antibody cocktail was compared with commercially available anti-SARS-CoV-2 S1 (anti-S1) and anti-S2 monoclonal antibodies. After optimization and calibration, the limit of detection (LOD) determination demonstrated a LOD = 0.53–0.75 ng/mL for the antibody cocktail-based sensor compared with 0.93 ng/mL and 0.99 ng/mL for the platforms using anti-S1 and anti-S2, respectively. The platforms were tested with human nasopharyngeal swab samples pre-diagnosed with RT-PCR (10 negatives and 40 positive samples). The positive samples include the original, alpha, beta, and delta variants (n = 10, for each). The polyclonal antibody cocktail performed better than commercial anti-S1 and anti-S2 antibodies for all samples reaching 100% overall sensitivity, specificity, and accuracy. It also showed a wide range of variants detection compared to monoclonal antibody-based platforms. The present work proposes a versatile electrochemical biosensor for the indiscriminate detection of the different variants of SARS-CoV-2 using a polyclonal antibody cocktail. Such diagnostic tools allowing the detection of variants can be of great efficiency and economic value in the fight against the ever-changing SARS-CoV-2 virus.
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spelling pubmed-88911552022-03-04 Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing Durmus, Ceren Balaban Hanoglu, Simge Harmanci, Duygu Moulahoum, Hichem Tok, Kerem Ghorbanizamani, Faezeh Sanli, Serdar Zihnioglu, Figen Evran, Serap Cicek, Candan Sertoz, Ruchan Arda, Bilgin Goksel, Tuncay Turhan, Kutsal Timur, Suna Talanta Article The increasing mutation frequency of the SARS-CoV-2 virus and the emergence of successive variants have made correct diagnosis hard to perform. Developing efficient and accurate methods to diagnose infected patients is crucial to effectively mitigate the pandemic. Here, we developed an electrochemical immunosensor based on SARS-CoV-2 antibody cocktail-conjugated magnetic nanoparticles for the sensitive and accurate detection of the SARS-CoV-2 virus and its variants in nasopharyngeal swabs. The application of the antibody cocktail was compared with commercially available anti-SARS-CoV-2 S1 (anti-S1) and anti-S2 monoclonal antibodies. After optimization and calibration, the limit of detection (LOD) determination demonstrated a LOD = 0.53–0.75 ng/mL for the antibody cocktail-based sensor compared with 0.93 ng/mL and 0.99 ng/mL for the platforms using anti-S1 and anti-S2, respectively. The platforms were tested with human nasopharyngeal swab samples pre-diagnosed with RT-PCR (10 negatives and 40 positive samples). The positive samples include the original, alpha, beta, and delta variants (n = 10, for each). The polyclonal antibody cocktail performed better than commercial anti-S1 and anti-S2 antibodies for all samples reaching 100% overall sensitivity, specificity, and accuracy. It also showed a wide range of variants detection compared to monoclonal antibody-based platforms. The present work proposes a versatile electrochemical biosensor for the indiscriminate detection of the different variants of SARS-CoV-2 using a polyclonal antibody cocktail. Such diagnostic tools allowing the detection of variants can be of great efficiency and economic value in the fight against the ever-changing SARS-CoV-2 virus. Elsevier B.V. 2022-06-01 2022-03-03 /pmc/articles/PMC8891155/ /pubmed/35248943 http://dx.doi.org/10.1016/j.talanta.2022.123356 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Durmus, Ceren
Balaban Hanoglu, Simge
Harmanci, Duygu
Moulahoum, Hichem
Tok, Kerem
Ghorbanizamani, Faezeh
Sanli, Serdar
Zihnioglu, Figen
Evran, Serap
Cicek, Candan
Sertoz, Ruchan
Arda, Bilgin
Goksel, Tuncay
Turhan, Kutsal
Timur, Suna
Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing
title Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing
title_full Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing
title_fullStr Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing
title_full_unstemmed Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing
title_short Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing
title_sort indiscriminate sars-cov-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891155/
https://www.ncbi.nlm.nih.gov/pubmed/35248943
http://dx.doi.org/10.1016/j.talanta.2022.123356
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