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Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow

Thrombus components are dynamically influenced by local blood flow and blood immune cells. After a large-vessel occlusion stroke, changes in the cerebral thrombus are unclear. Here we assessed a total of 206 cerebral thrombi from patients with ischemic stroke undergoing endovascular thrombectomy. Th...

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Autores principales: Chen, Xi, Wang, Li, Jiang, Meiling, Lin, Lin, Ba, Zhaojing, Tian, Hao, Li, Guangjian, Chen, Lin, Liu, Qu, Hou, Xianhua, Wu, Min, Liu, Lu, Ju, Wenying, Zeng, Wen, Zhou, Zhenhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891436/
https://www.ncbi.nlm.nih.gov/pubmed/35251025
http://dx.doi.org/10.3389/fimmu.2022.834562
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author Chen, Xi
Wang, Li
Jiang, Meiling
Lin, Lin
Ba, Zhaojing
Tian, Hao
Li, Guangjian
Chen, Lin
Liu, Qu
Hou, Xianhua
Wu, Min
Liu, Lu
Ju, Wenying
Zeng, Wen
Zhou, Zhenhua
author_facet Chen, Xi
Wang, Li
Jiang, Meiling
Lin, Lin
Ba, Zhaojing
Tian, Hao
Li, Guangjian
Chen, Lin
Liu, Qu
Hou, Xianhua
Wu, Min
Liu, Lu
Ju, Wenying
Zeng, Wen
Zhou, Zhenhua
author_sort Chen, Xi
collection PubMed
description Thrombus components are dynamically influenced by local blood flow and blood immune cells. After a large-vessel occlusion stroke, changes in the cerebral thrombus are unclear. Here we assessed a total of 206 cerebral thrombi from patients with ischemic stroke undergoing endovascular thrombectomy. The thrombi were categorized by time to reperfusion of <4 h (T4), 4–8 h (T4–8), and >8 h (T8). The cellular compositions in thrombus were analyzed, and relevant clinical features were compared. Both white blood cells and neutrophils were increased and then decreased in thrombus with time to reperfusion, which were positively correlated with those in peripheral blood. The neutrophil extracellular trap (NET) content in thrombus was correlated with the degree of neurological impairment of patients. Moreover, with prolonged time to reperfusion, the patients showed a trend of better collateral grade, which was associated with a lower NET content in the thrombus. In conclusion, the present results reveal the relationship between time-related endovascular immune response and clinical symptoms post-stroke from the perspective of thrombus and peripheral blood. The time-related pathological changes of cerebral thrombus may not be the direct cause for the difficulty in thrombolysis and thrombectomy. A low NET content in thrombi indicates excellent collateral flow, which suggests that treatments targeting NETs in thrombi might be beneficial for early neurological protection.
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spelling pubmed-88914362022-03-04 Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow Chen, Xi Wang, Li Jiang, Meiling Lin, Lin Ba, Zhaojing Tian, Hao Li, Guangjian Chen, Lin Liu, Qu Hou, Xianhua Wu, Min Liu, Lu Ju, Wenying Zeng, Wen Zhou, Zhenhua Front Immunol Immunology Thrombus components are dynamically influenced by local blood flow and blood immune cells. After a large-vessel occlusion stroke, changes in the cerebral thrombus are unclear. Here we assessed a total of 206 cerebral thrombi from patients with ischemic stroke undergoing endovascular thrombectomy. The thrombi were categorized by time to reperfusion of <4 h (T4), 4–8 h (T4–8), and >8 h (T8). The cellular compositions in thrombus were analyzed, and relevant clinical features were compared. Both white blood cells and neutrophils were increased and then decreased in thrombus with time to reperfusion, which were positively correlated with those in peripheral blood. The neutrophil extracellular trap (NET) content in thrombus was correlated with the degree of neurological impairment of patients. Moreover, with prolonged time to reperfusion, the patients showed a trend of better collateral grade, which was associated with a lower NET content in the thrombus. In conclusion, the present results reveal the relationship between time-related endovascular immune response and clinical symptoms post-stroke from the perspective of thrombus and peripheral blood. The time-related pathological changes of cerebral thrombus may not be the direct cause for the difficulty in thrombolysis and thrombectomy. A low NET content in thrombi indicates excellent collateral flow, which suggests that treatments targeting NETs in thrombi might be beneficial for early neurological protection. Frontiers Media S.A. 2022-02-17 /pmc/articles/PMC8891436/ /pubmed/35251025 http://dx.doi.org/10.3389/fimmu.2022.834562 Text en Copyright © 2022 Chen, Wang, Jiang, Lin, Ba, Tian, Li, Chen, Liu, Hou, Wu, Liu, Ju, Zeng and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Chen, Xi
Wang, Li
Jiang, Meiling
Lin, Lin
Ba, Zhaojing
Tian, Hao
Li, Guangjian
Chen, Lin
Liu, Qu
Hou, Xianhua
Wu, Min
Liu, Lu
Ju, Wenying
Zeng, Wen
Zhou, Zhenhua
Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow
title Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow
title_full Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow
title_fullStr Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow
title_full_unstemmed Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow
title_short Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow
title_sort leukocytes in cerebral thrombus respond to large-vessel occlusion in a time-dependent manner and the association of nets with collateral flow
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891436/
https://www.ncbi.nlm.nih.gov/pubmed/35251025
http://dx.doi.org/10.3389/fimmu.2022.834562
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