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Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation

Phaeocystis globosa (P. globosa) is one of the dominant algae during harmful algal blooms (HABs) in coastal regions of Southern China. P. globosa exhibits complex heteromorphic life cycles that could switch between solitary cells and colonies. The ecological success of P. globosa has been attributed...

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Autores principales: Xu, Shuaishuai, Wang, Xiaodong, Liu, Jie, Zhou, Fengli, Guo, Kangli, Chen, Songze, Wang, Zhao-hui, Wang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891983/
https://www.ncbi.nlm.nih.gov/pubmed/35250943
http://dx.doi.org/10.3389/fmicb.2022.826602
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author Xu, Shuaishuai
Wang, Xiaodong
Liu, Jie
Zhou, Fengli
Guo, Kangli
Chen, Songze
Wang, Zhao-hui
Wang, Yan
author_facet Xu, Shuaishuai
Wang, Xiaodong
Liu, Jie
Zhou, Fengli
Guo, Kangli
Chen, Songze
Wang, Zhao-hui
Wang, Yan
author_sort Xu, Shuaishuai
collection PubMed
description Phaeocystis globosa (P. globosa) is one of the dominant algae during harmful algal blooms (HABs) in coastal regions of Southern China. P. globosa exhibits complex heteromorphic life cycles that could switch between solitary cells and colonies. The ecological success of P. globosa has been attributed to its colony formation, although underlying mechanisms remain unknown. Here, we investigated different bacterial communities associated with P. globosa colonies and their influence on colony formation of two P. globosa strains isolated from coastal waters of Guangxi (GX) and Shantou (ST). Eight operational taxonomic units (OTUs) were observed in ST co-cultures and were identified as biomarkers based on Linear discriminant analysis Effect Size (LEfSe) analysis, while seven biomarkers were identified in P. globosa GX co-cultures. Bacterial communities associated with the P. globosa GX were more diverse than those of the ST strain. The most dominant phylum in the two co-cultures was Proteobacteria, within which Marinobacter was the most abundant genus in both GX and ST co-cultures. Bacteroidota were only observed in the GX co-cultures and Planctomycetota were only observed in the ST co-cultures. Co-culture experiments revealed that P. globosa colony formation was not influenced by low and medium cell densities of Marinobacter sp. GS7, but was inhibited by high cell densities of Marinobacter sp. GS7. Overall, these results indicated that the associated bacteria are selected by different P. globosa strains, which may affect the colony formation and development of P. globosa.
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spelling pubmed-88919832022-03-04 Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation Xu, Shuaishuai Wang, Xiaodong Liu, Jie Zhou, Fengli Guo, Kangli Chen, Songze Wang, Zhao-hui Wang, Yan Front Microbiol Microbiology Phaeocystis globosa (P. globosa) is one of the dominant algae during harmful algal blooms (HABs) in coastal regions of Southern China. P. globosa exhibits complex heteromorphic life cycles that could switch between solitary cells and colonies. The ecological success of P. globosa has been attributed to its colony formation, although underlying mechanisms remain unknown. Here, we investigated different bacterial communities associated with P. globosa colonies and their influence on colony formation of two P. globosa strains isolated from coastal waters of Guangxi (GX) and Shantou (ST). Eight operational taxonomic units (OTUs) were observed in ST co-cultures and were identified as biomarkers based on Linear discriminant analysis Effect Size (LEfSe) analysis, while seven biomarkers were identified in P. globosa GX co-cultures. Bacterial communities associated with the P. globosa GX were more diverse than those of the ST strain. The most dominant phylum in the two co-cultures was Proteobacteria, within which Marinobacter was the most abundant genus in both GX and ST co-cultures. Bacteroidota were only observed in the GX co-cultures and Planctomycetota were only observed in the ST co-cultures. Co-culture experiments revealed that P. globosa colony formation was not influenced by low and medium cell densities of Marinobacter sp. GS7, but was inhibited by high cell densities of Marinobacter sp. GS7. Overall, these results indicated that the associated bacteria are selected by different P. globosa strains, which may affect the colony formation and development of P. globosa. Frontiers Media S.A. 2022-02-17 /pmc/articles/PMC8891983/ /pubmed/35250943 http://dx.doi.org/10.3389/fmicb.2022.826602 Text en Copyright © 2022 Xu, Wang, Liu, Zhou, Guo, Chen, Wang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xu, Shuaishuai
Wang, Xiaodong
Liu, Jie
Zhou, Fengli
Guo, Kangli
Chen, Songze
Wang, Zhao-hui
Wang, Yan
Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation
title Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation
title_full Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation
title_fullStr Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation
title_full_unstemmed Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation
title_short Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation
title_sort bacteria associated with phaeocystis globosa and their influence on colony formation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891983/
https://www.ncbi.nlm.nih.gov/pubmed/35250943
http://dx.doi.org/10.3389/fmicb.2022.826602
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