Highly multiplex PCR assays by coupling the 5′-flap endonuclease activity of Taq DNA polymerase and molecular beacon reporters

Real-time PCR is the most utilized nucleic acid testing tool in clinical settings. However, the number of targets detectable per reaction are restricted by current modes. Here, we describe a single-step, multiplex approach capable of detecting dozens of targets per reaction in a real-time PCR therma...

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Detalles Bibliográficos
Autores principales: Huang, Qiuying, Chen, Dongmei, Du, Chen, Liu, Qiaoqiao, Lin, Su, Liang, Lanlan, Xu, Ye, Liao, Yiqun, Li, Qingge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8892341/
https://www.ncbi.nlm.nih.gov/pubmed/35197282
http://dx.doi.org/10.1073/pnas.2110672119

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