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Challenges and approaches to studying pore-forming proteins
Pore-forming proteins (PFPs) are a broad class of molecules that comprise various families, structural folds, and assembly pathways. In nature, PFPs are most often deployed by their host organisms to defend against other organisms. In humans, this is apparent in the immune system, where several immu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8892993/ https://www.ncbi.nlm.nih.gov/pubmed/34747994 http://dx.doi.org/10.1042/BST20210706 |
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author | Benton, Joshua T. Bayly-Jones, Charles |
author_facet | Benton, Joshua T. Bayly-Jones, Charles |
author_sort | Benton, Joshua T. |
collection | PubMed |
description | Pore-forming proteins (PFPs) are a broad class of molecules that comprise various families, structural folds, and assembly pathways. In nature, PFPs are most often deployed by their host organisms to defend against other organisms. In humans, this is apparent in the immune system, where several immune effectors possess pore-forming activity. Furthermore, applications of PFPs are found in next-generation low-cost DNA sequencing, agricultural crop protection, pest control, and biosensing. The advent of cryoEM has propelled the field forward. Nevertheless, significant challenges and knowledge-gaps remain. Overcoming these challenges is particularly important for the development of custom, purpose-engineered PFPs with novel or desired properties. Emerging single-molecule techniques and methods are helping to address these unanswered questions. Here we review the current challenges, problems, and approaches to studying PFPs. |
format | Online Article Text |
id | pubmed-8892993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88929932022-03-10 Challenges and approaches to studying pore-forming proteins Benton, Joshua T. Bayly-Jones, Charles Biochem Soc Trans Review Articles Pore-forming proteins (PFPs) are a broad class of molecules that comprise various families, structural folds, and assembly pathways. In nature, PFPs are most often deployed by their host organisms to defend against other organisms. In humans, this is apparent in the immune system, where several immune effectors possess pore-forming activity. Furthermore, applications of PFPs are found in next-generation low-cost DNA sequencing, agricultural crop protection, pest control, and biosensing. The advent of cryoEM has propelled the field forward. Nevertheless, significant challenges and knowledge-gaps remain. Overcoming these challenges is particularly important for the development of custom, purpose-engineered PFPs with novel or desired properties. Emerging single-molecule techniques and methods are helping to address these unanswered questions. Here we review the current challenges, problems, and approaches to studying PFPs. Portland Press Ltd. 2021-12-17 2021-11-08 /pmc/articles/PMC8892993/ /pubmed/34747994 http://dx.doi.org/10.1042/BST20210706 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of Monash University in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with CAUL. |
spellingShingle | Review Articles Benton, Joshua T. Bayly-Jones, Charles Challenges and approaches to studying pore-forming proteins |
title | Challenges and approaches to studying pore-forming proteins |
title_full | Challenges and approaches to studying pore-forming proteins |
title_fullStr | Challenges and approaches to studying pore-forming proteins |
title_full_unstemmed | Challenges and approaches to studying pore-forming proteins |
title_short | Challenges and approaches to studying pore-forming proteins |
title_sort | challenges and approaches to studying pore-forming proteins |
topic | Review Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8892993/ https://www.ncbi.nlm.nih.gov/pubmed/34747994 http://dx.doi.org/10.1042/BST20210706 |
work_keys_str_mv | AT bentonjoshuat challengesandapproachestostudyingporeformingproteins AT baylyjonescharles challengesandapproachestostudyingporeformingproteins |