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Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus

Confirmed SARS-coronavirus-2 infection with gastrointestinal symptoms and changes in microbiota associated with coronavirus disease 2019 (COVID-19) severity have been previously reported, but the disease impact on the architecture and cellularity of ileal Peyer’s patches (PP) remains unknown. Here w...

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Autores principales: Trevelin, Silvia C., Pickering, Suzanne, Todd, Katrina, Bishop, Cynthia, Pitcher, Michael, Garrido Mesa, Jose, Montorsi, Lucia, Spada, Filomena, Petrov, Nedyalko, Green, Anna, Shankar-Hari, Manu, Neil, Stuart J.D., Spencer, Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8893224/
https://www.ncbi.nlm.nih.gov/pubmed/35251032
http://dx.doi.org/10.3389/fimmu.2022.838328
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author Trevelin, Silvia C.
Pickering, Suzanne
Todd, Katrina
Bishop, Cynthia
Pitcher, Michael
Garrido Mesa, Jose
Montorsi, Lucia
Spada, Filomena
Petrov, Nedyalko
Green, Anna
Shankar-Hari, Manu
Neil, Stuart J.D.
Spencer, Jo
author_facet Trevelin, Silvia C.
Pickering, Suzanne
Todd, Katrina
Bishop, Cynthia
Pitcher, Michael
Garrido Mesa, Jose
Montorsi, Lucia
Spada, Filomena
Petrov, Nedyalko
Green, Anna
Shankar-Hari, Manu
Neil, Stuart J.D.
Spencer, Jo
author_sort Trevelin, Silvia C.
collection PubMed
description Confirmed SARS-coronavirus-2 infection with gastrointestinal symptoms and changes in microbiota associated with coronavirus disease 2019 (COVID-19) severity have been previously reported, but the disease impact on the architecture and cellularity of ileal Peyer’s patches (PP) remains unknown. Here we analysed post-mortem tissues from throughout the gastrointestinal (GI) tract of patients who died with COVID-19. When virus was detected by PCR in the GI tract, immunohistochemistry identified virus in epithelium and lamina propria macrophages, but not in lymphoid tissues. Immunohistochemistry and imaging mass cytometry (IMC) analysis of ileal PP revealed depletion of germinal centres (GC), disruption of B cell/T cell zonation and decreased potential B and T cell interaction and lower nuclear density in COVID-19 patients. This occurred independent of the local viral levels. The changes in PP demonstrate that the ability to mount an intestinal immune response is compromised in severe COVID-19, which could contribute to observed dysbiosis.
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spelling pubmed-88932242022-03-04 Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus Trevelin, Silvia C. Pickering, Suzanne Todd, Katrina Bishop, Cynthia Pitcher, Michael Garrido Mesa, Jose Montorsi, Lucia Spada, Filomena Petrov, Nedyalko Green, Anna Shankar-Hari, Manu Neil, Stuart J.D. Spencer, Jo Front Immunol Immunology Confirmed SARS-coronavirus-2 infection with gastrointestinal symptoms and changes in microbiota associated with coronavirus disease 2019 (COVID-19) severity have been previously reported, but the disease impact on the architecture and cellularity of ileal Peyer’s patches (PP) remains unknown. Here we analysed post-mortem tissues from throughout the gastrointestinal (GI) tract of patients who died with COVID-19. When virus was detected by PCR in the GI tract, immunohistochemistry identified virus in epithelium and lamina propria macrophages, but not in lymphoid tissues. Immunohistochemistry and imaging mass cytometry (IMC) analysis of ileal PP revealed depletion of germinal centres (GC), disruption of B cell/T cell zonation and decreased potential B and T cell interaction and lower nuclear density in COVID-19 patients. This occurred independent of the local viral levels. The changes in PP demonstrate that the ability to mount an intestinal immune response is compromised in severe COVID-19, which could contribute to observed dysbiosis. Frontiers Media S.A. 2022-02-17 /pmc/articles/PMC8893224/ /pubmed/35251032 http://dx.doi.org/10.3389/fimmu.2022.838328 Text en Copyright © 2022 Trevelin, Pickering, Todd, Bishop, Pitcher, Garrido Mesa, Montorsi, Spada, Petrov, Green, Shankar-Hari, Neil and Spencer https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Trevelin, Silvia C.
Pickering, Suzanne
Todd, Katrina
Bishop, Cynthia
Pitcher, Michael
Garrido Mesa, Jose
Montorsi, Lucia
Spada, Filomena
Petrov, Nedyalko
Green, Anna
Shankar-Hari, Manu
Neil, Stuart J.D.
Spencer, Jo
Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus
title Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus
title_full Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus
title_fullStr Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus
title_full_unstemmed Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus
title_short Disrupted Peyer’s Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus
title_sort disrupted peyer’s patch microanatomy in covid-19 including germinal centre atrophy independent of local virus
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8893224/
https://www.ncbi.nlm.nih.gov/pubmed/35251032
http://dx.doi.org/10.3389/fimmu.2022.838328
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