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Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer

Efficient screening of anticancer agents is in urgent need to develop new drugs that combat malignant tumors and drug resistance. In this study, a combined strategy composed by solvent partition and HPLC fractionation was developed to generate an herbal fraction library of Salviae Miltiorrhizae Radi...

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Autores principales: Ye, Qing-Mei, Ji, Xiaozhen, Wang, Bin, Yu, Miao, Cai, Jin, Zeng, Weinv, Chen, Weikang, Han, Fangxuan, Huang, Guolei, Zheng, Caijuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894037/
https://www.ncbi.nlm.nih.gov/pubmed/35251179
http://dx.doi.org/10.1155/2022/9955834
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author Ye, Qing-Mei
Ji, Xiaozhen
Wang, Bin
Yu, Miao
Cai, Jin
Zeng, Weinv
Chen, Weikang
Han, Fangxuan
Huang, Guolei
Zheng, Caijuan
author_facet Ye, Qing-Mei
Ji, Xiaozhen
Wang, Bin
Yu, Miao
Cai, Jin
Zeng, Weinv
Chen, Weikang
Han, Fangxuan
Huang, Guolei
Zheng, Caijuan
author_sort Ye, Qing-Mei
collection PubMed
description Efficient screening of anticancer agents is in urgent need to develop new drugs that combat malignant tumors and drug resistance. In this study, a combined strategy composed by solvent partition and HPLC fractionation was developed to generate an herbal fraction library of Salviae Miltiorrhizae Radix et Rhizoma to quickly and efficiently screen anticancer agents. All library entries are directed into 96 well plates which are well mapped with HPLC chromatograms. The cell proliferation assay revealed seven active subfractions. Then, the major active ten peaks in these subfractions were prepared and isolated by semipreparative HPLC, and their inhibitory activities against prostate cancer cells were then tested at the same concentration level, leading to the identification of several active compounds. In addition, the structures of compounds arucadiol (2), 15,16-dihydrotanshinone I (4), methyl tanshinonate (5), cryptanshinone (7), 1,2-dihydrotanshinquinone I (9), and tanshinone IIA (10) were characterized by mass spectrometry and X-ray crystallographic analysis, and they were confirmed to be active in suppressing prostate cancer cell proliferation at 7.5 or 15 μg/mL, among which, the minor compounds 2, 4, and 5 showed higher activities than 9 and 10. This study provided a rapid strategy of identifying new anticancer agents in Salviae Miltiorrhizae Radix et Rhizoma, which can be applied in other herbal medicines.
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spelling pubmed-88940372022-03-04 Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer Ye, Qing-Mei Ji, Xiaozhen Wang, Bin Yu, Miao Cai, Jin Zeng, Weinv Chen, Weikang Han, Fangxuan Huang, Guolei Zheng, Caijuan J Oncol Research Article Efficient screening of anticancer agents is in urgent need to develop new drugs that combat malignant tumors and drug resistance. In this study, a combined strategy composed by solvent partition and HPLC fractionation was developed to generate an herbal fraction library of Salviae Miltiorrhizae Radix et Rhizoma to quickly and efficiently screen anticancer agents. All library entries are directed into 96 well plates which are well mapped with HPLC chromatograms. The cell proliferation assay revealed seven active subfractions. Then, the major active ten peaks in these subfractions were prepared and isolated by semipreparative HPLC, and their inhibitory activities against prostate cancer cells were then tested at the same concentration level, leading to the identification of several active compounds. In addition, the structures of compounds arucadiol (2), 15,16-dihydrotanshinone I (4), methyl tanshinonate (5), cryptanshinone (7), 1,2-dihydrotanshinquinone I (9), and tanshinone IIA (10) were characterized by mass spectrometry and X-ray crystallographic analysis, and they were confirmed to be active in suppressing prostate cancer cell proliferation at 7.5 or 15 μg/mL, among which, the minor compounds 2, 4, and 5 showed higher activities than 9 and 10. This study provided a rapid strategy of identifying new anticancer agents in Salviae Miltiorrhizae Radix et Rhizoma, which can be applied in other herbal medicines. Hindawi 2022-02-24 /pmc/articles/PMC8894037/ /pubmed/35251179 http://dx.doi.org/10.1155/2022/9955834 Text en Copyright © 2022 Qing-Mei Ye et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ye, Qing-Mei
Ji, Xiaozhen
Wang, Bin
Yu, Miao
Cai, Jin
Zeng, Weinv
Chen, Weikang
Han, Fangxuan
Huang, Guolei
Zheng, Caijuan
Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer
title Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer
title_full Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer
title_fullStr Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer
title_full_unstemmed Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer
title_short Construction and Screening of Fractional Library of Salviae Miltiorrhizae Radix et Rhizoma for the Rapid Identification of Active Compounds against Prostate Cancer
title_sort construction and screening of fractional library of salviae miltiorrhizae radix et rhizoma for the rapid identification of active compounds against prostate cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894037/
https://www.ncbi.nlm.nih.gov/pubmed/35251179
http://dx.doi.org/10.1155/2022/9955834
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