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Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum

Some plants abandoned photosynthesis and developed full dependency on fungi for nutrition. Most of the so-called mycoheterotrophic plants exhibit high specificity towards their fungal partners. We tested whether natural rarity of mycoheterotrophic plants and usual small and fluctuating population si...

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Autores principales: Minasiewicz, Julita, Krawczyk, Emilia, Znaniecka, Joanna, Vincenot, Lucie, Zheleznaya, Ekaterina, Korybut-Orlowska, Joanna, Kull, Tiiu, Selosse, Marc-André
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894228/
https://www.ncbi.nlm.nih.gov/pubmed/34993702
http://dx.doi.org/10.1007/s10265-021-01364-7
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author Minasiewicz, Julita
Krawczyk, Emilia
Znaniecka, Joanna
Vincenot, Lucie
Zheleznaya, Ekaterina
Korybut-Orlowska, Joanna
Kull, Tiiu
Selosse, Marc-André
author_facet Minasiewicz, Julita
Krawczyk, Emilia
Znaniecka, Joanna
Vincenot, Lucie
Zheleznaya, Ekaterina
Korybut-Orlowska, Joanna
Kull, Tiiu
Selosse, Marc-André
author_sort Minasiewicz, Julita
collection PubMed
description Some plants abandoned photosynthesis and developed full dependency on fungi for nutrition. Most of the so-called mycoheterotrophic plants exhibit high specificity towards their fungal partners. We tested whether natural rarity of mycoheterotrophic plants and usual small and fluctuating population size make their populations more prone to genetic differentiation caused by restricted gene flow and/or genetic drift. We also tested whether these genetic characteristics might in turn shape divergent fungal preferences. We studied the mycoheterotrophic orchid Epipogium aphyllum, addressing the joint issues of genetic structure of its populations over Europe and possible consequences for mycorrhizal specificity within the associated fungal taxa. Out of 27 sampled E. aphyllum populations, nine were included for genetic diversity assessment using nine nuclear microsatellites and plastid DNA. Population genetic structure was inferred based on the total number of populations. Individuals from 17 locations were included into analysis of genetic identity of mycorrhizal fungi of E. aphyllum based on barcoding by nuclear ribosomal DNA. Epipogium aphyllum populations revealed high genetic diversity (uHe = 0.562) and low genetic differentiation over vast distances (F(ST) = 0.106 for nuclear microsatellites and F(ST) = 0.156 for plastid DNA). Bayesian clustering analyses identified only two genetic clusters, with a high degree of admixture. Epipogium aphyllum genets arise from panmixia and display locally variable, but relatively high production of ramets, as shown by a low value of rarefied genotypic richness (R(r) = 0.265). Epipogium aphyllum genotype control over partner selection was negligible as (1) we found ramets from a single genetic individual associated with up to 68% of the known Inocybe spp. associating with the plant species, (2) and partner identity did not show any geographic structure. The absence of mosaicism in the mycorrhizal specificity over Europe may be linked to preferential allogamous habit of E. aphyllum and significant gene flow, which tend to promote host generalism. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10265-021-01364-7.
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spelling pubmed-88942282022-03-08 Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum Minasiewicz, Julita Krawczyk, Emilia Znaniecka, Joanna Vincenot, Lucie Zheleznaya, Ekaterina Korybut-Orlowska, Joanna Kull, Tiiu Selosse, Marc-André J Plant Res Regular Paper – Genetics/Developmental Biology Some plants abandoned photosynthesis and developed full dependency on fungi for nutrition. Most of the so-called mycoheterotrophic plants exhibit high specificity towards their fungal partners. We tested whether natural rarity of mycoheterotrophic plants and usual small and fluctuating population size make their populations more prone to genetic differentiation caused by restricted gene flow and/or genetic drift. We also tested whether these genetic characteristics might in turn shape divergent fungal preferences. We studied the mycoheterotrophic orchid Epipogium aphyllum, addressing the joint issues of genetic structure of its populations over Europe and possible consequences for mycorrhizal specificity within the associated fungal taxa. Out of 27 sampled E. aphyllum populations, nine were included for genetic diversity assessment using nine nuclear microsatellites and plastid DNA. Population genetic structure was inferred based on the total number of populations. Individuals from 17 locations were included into analysis of genetic identity of mycorrhizal fungi of E. aphyllum based on barcoding by nuclear ribosomal DNA. Epipogium aphyllum populations revealed high genetic diversity (uHe = 0.562) and low genetic differentiation over vast distances (F(ST) = 0.106 for nuclear microsatellites and F(ST) = 0.156 for plastid DNA). Bayesian clustering analyses identified only two genetic clusters, with a high degree of admixture. Epipogium aphyllum genets arise from panmixia and display locally variable, but relatively high production of ramets, as shown by a low value of rarefied genotypic richness (R(r) = 0.265). Epipogium aphyllum genotype control over partner selection was negligible as (1) we found ramets from a single genetic individual associated with up to 68% of the known Inocybe spp. associating with the plant species, (2) and partner identity did not show any geographic structure. The absence of mosaicism in the mycorrhizal specificity over Europe may be linked to preferential allogamous habit of E. aphyllum and significant gene flow, which tend to promote host generalism. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10265-021-01364-7. Springer Singapore 2022-01-06 2022 /pmc/articles/PMC8894228/ /pubmed/34993702 http://dx.doi.org/10.1007/s10265-021-01364-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Regular Paper – Genetics/Developmental Biology
Minasiewicz, Julita
Krawczyk, Emilia
Znaniecka, Joanna
Vincenot, Lucie
Zheleznaya, Ekaterina
Korybut-Orlowska, Joanna
Kull, Tiiu
Selosse, Marc-André
Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum
title Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum
title_full Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum
title_fullStr Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum
title_full_unstemmed Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum
title_short Weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid Epipogium aphyllum
title_sort weak population spatial genetic structure and low infraspecific specificity for fungal partners in the rare mycoheterotrophic orchid epipogium aphyllum
topic Regular Paper – Genetics/Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894228/
https://www.ncbi.nlm.nih.gov/pubmed/34993702
http://dx.doi.org/10.1007/s10265-021-01364-7
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