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Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells

The performance of implanted biomaterials is largely determined by their interaction with the host immune system. As a fibrous-like 3D network, fibrin matrix formed at the interfaces of tissue and material, whose effects on dendritic cells (DCs) remain unknown. Here, a bone plates implantation model...

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Autores principales: Hu, Wenhui, Wang, Yun, Chen, Jin, Yu, Peng, Tang, Fuzhou, Hu, Zuquan, Zhou, Jing, Liu, Lina, Qiu, Wei, Ye, Yuannong, Jia, Yi, Zhou, Shi, Long, Jinhua, Zeng, Zhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894278/
https://www.ncbi.nlm.nih.gov/pubmed/35252832
http://dx.doi.org/10.1016/j.mtbio.2022.100224
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author Hu, Wenhui
Wang, Yun
Chen, Jin
Yu, Peng
Tang, Fuzhou
Hu, Zuquan
Zhou, Jing
Liu, Lina
Qiu, Wei
Ye, Yuannong
Jia, Yi
Zhou, Shi
Long, Jinhua
Zeng, Zhu
author_facet Hu, Wenhui
Wang, Yun
Chen, Jin
Yu, Peng
Tang, Fuzhou
Hu, Zuquan
Zhou, Jing
Liu, Lina
Qiu, Wei
Ye, Yuannong
Jia, Yi
Zhou, Shi
Long, Jinhua
Zeng, Zhu
author_sort Hu, Wenhui
collection PubMed
description The performance of implanted biomaterials is largely determined by their interaction with the host immune system. As a fibrous-like 3D network, fibrin matrix formed at the interfaces of tissue and material, whose effects on dendritic cells (DCs) remain unknown. Here, a bone plates implantation model was developed to evaluate the fibrin matrix deposition and DCs recruitment in vivo. The DCs responses to fibrin matrix were further analyzed by a 2D and 3D fibrin matrix model in vitro. In vivo results indicated that large amount of fibrin matrix deposited on the interface between the tissue and bone plates, where DCs were recruited. Subsequent in vitro testing denoted that DCs underwent significant shape deformation and cytoskeleton reorganization, as well as mechanical property alteration. Furthermore, the immune function of imDCs and mDCs were negatively and positively regulated, respectively. The underlying mechano-immunology coupling mechanisms involved RhoA and CDC42 signaling pathways. These results suggested that fibrin plays a key role in regulating DCs immunological behaviors, providing a valuable immunomodulatory strategy for tissue healing, regeneration and implantation.
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spelling pubmed-88942782022-03-05 Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells Hu, Wenhui Wang, Yun Chen, Jin Yu, Peng Tang, Fuzhou Hu, Zuquan Zhou, Jing Liu, Lina Qiu, Wei Ye, Yuannong Jia, Yi Zhou, Shi Long, Jinhua Zeng, Zhu Mater Today Bio Full Length Article The performance of implanted biomaterials is largely determined by their interaction with the host immune system. As a fibrous-like 3D network, fibrin matrix formed at the interfaces of tissue and material, whose effects on dendritic cells (DCs) remain unknown. Here, a bone plates implantation model was developed to evaluate the fibrin matrix deposition and DCs recruitment in vivo. The DCs responses to fibrin matrix were further analyzed by a 2D and 3D fibrin matrix model in vitro. In vivo results indicated that large amount of fibrin matrix deposited on the interface between the tissue and bone plates, where DCs were recruited. Subsequent in vitro testing denoted that DCs underwent significant shape deformation and cytoskeleton reorganization, as well as mechanical property alteration. Furthermore, the immune function of imDCs and mDCs were negatively and positively regulated, respectively. The underlying mechano-immunology coupling mechanisms involved RhoA and CDC42 signaling pathways. These results suggested that fibrin plays a key role in regulating DCs immunological behaviors, providing a valuable immunomodulatory strategy for tissue healing, regeneration and implantation. Elsevier 2022-02-26 /pmc/articles/PMC8894278/ /pubmed/35252832 http://dx.doi.org/10.1016/j.mtbio.2022.100224 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Hu, Wenhui
Wang, Yun
Chen, Jin
Yu, Peng
Tang, Fuzhou
Hu, Zuquan
Zhou, Jing
Liu, Lina
Qiu, Wei
Ye, Yuannong
Jia, Yi
Zhou, Shi
Long, Jinhua
Zeng, Zhu
Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells
title Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells
title_full Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells
title_fullStr Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells
title_full_unstemmed Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells
title_short Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells
title_sort regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894278/
https://www.ncbi.nlm.nih.gov/pubmed/35252832
http://dx.doi.org/10.1016/j.mtbio.2022.100224
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