Cargando…
Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication
Foot-and-mouth disease virus (FMDV) is a highly contagious viral disease that mainly infects cloven-hoofed animals. Propagation of FMDV by cell culture is an important method to preserve viral biological and antigenic characteristics, which is crucial in FMD monitoring and vaccine production. Howeve...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894894/ https://www.ncbi.nlm.nih.gov/pubmed/35250927 http://dx.doi.org/10.3389/fmicb.2022.805606 |
_version_ | 1784662788799463424 |
---|---|
author | Hou, Shitong Wang, Xiangwei Ren, Shanhui Meng, Xuelian Yin, Xiangping Zhang, Jie Tarasiuk, Kazimierz Pejsak, Zygmunt Jiang, Tao Mao, Ruoqing Zhang, Yongguang Sun, Yuefeng |
author_facet | Hou, Shitong Wang, Xiangwei Ren, Shanhui Meng, Xuelian Yin, Xiangping Zhang, Jie Tarasiuk, Kazimierz Pejsak, Zygmunt Jiang, Tao Mao, Ruoqing Zhang, Yongguang Sun, Yuefeng |
author_sort | Hou, Shitong |
collection | PubMed |
description | Foot-and-mouth disease virus (FMDV) is a highly contagious viral disease that mainly infects cloven-hoofed animals. Propagation of FMDV by cell culture is an important method to preserve viral biological and antigenic characteristics, which is crucial in FMD monitoring and vaccine production. However, only a few cell lines are sensitive to FMDV, and there is still a lot of room for improvement. Acetylation is an important post-translational modification, which is dynamically regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). However, the study of the relationship between FMDV and HDACs is still unclear. HDAC9 belongs to the class II of HDACs family; in this study, HDAC9 knockout (KO) BHK-21 cells were successfully established using CRISPR/cas9 technology. The results of karyotype analysis, growth curve analysis, and morphological observation showed that the HDAC9 knockout cell line was stable in growth and morphological characteristics. After infection with FMDV, the expression of viral RNA and protein, viral titers, and the copies of viral RNA in HDAC9-KO cells were significantly higher than those in NC cells. Meanwhile, RNA-seq technology was used to sequence HDAC9-KO cells and NC cells infected and uninfected with FMDV. It was found that the differentially expressed innate immune factors containing NFKBIA, SOD2, IL2RG, BCL2L1, CXCL1/2/3, and IL1RAP have significantly enriched in the Jak-STAT, NOD-like receptor, Toll-like receptor, NF-κB, and MAPK signaling pathway. RT-qPCR was performed to detect the expression level of differentially expressed genes and showed consistency with the RNA-seq data. These results preliminarily reveal the role of HDAC9 in host antiviral innate immune response, and the HDAC9-KO cell line could also serve as a useful tool for FMDV research. |
format | Online Article Text |
id | pubmed-8894894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88948942022-03-05 Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication Hou, Shitong Wang, Xiangwei Ren, Shanhui Meng, Xuelian Yin, Xiangping Zhang, Jie Tarasiuk, Kazimierz Pejsak, Zygmunt Jiang, Tao Mao, Ruoqing Zhang, Yongguang Sun, Yuefeng Front Microbiol Microbiology Foot-and-mouth disease virus (FMDV) is a highly contagious viral disease that mainly infects cloven-hoofed animals. Propagation of FMDV by cell culture is an important method to preserve viral biological and antigenic characteristics, which is crucial in FMD monitoring and vaccine production. However, only a few cell lines are sensitive to FMDV, and there is still a lot of room for improvement. Acetylation is an important post-translational modification, which is dynamically regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). However, the study of the relationship between FMDV and HDACs is still unclear. HDAC9 belongs to the class II of HDACs family; in this study, HDAC9 knockout (KO) BHK-21 cells were successfully established using CRISPR/cas9 technology. The results of karyotype analysis, growth curve analysis, and morphological observation showed that the HDAC9 knockout cell line was stable in growth and morphological characteristics. After infection with FMDV, the expression of viral RNA and protein, viral titers, and the copies of viral RNA in HDAC9-KO cells were significantly higher than those in NC cells. Meanwhile, RNA-seq technology was used to sequence HDAC9-KO cells and NC cells infected and uninfected with FMDV. It was found that the differentially expressed innate immune factors containing NFKBIA, SOD2, IL2RG, BCL2L1, CXCL1/2/3, and IL1RAP have significantly enriched in the Jak-STAT, NOD-like receptor, Toll-like receptor, NF-κB, and MAPK signaling pathway. RT-qPCR was performed to detect the expression level of differentially expressed genes and showed consistency with the RNA-seq data. These results preliminarily reveal the role of HDAC9 in host antiviral innate immune response, and the HDAC9-KO cell line could also serve as a useful tool for FMDV research. Frontiers Media S.A. 2022-02-18 /pmc/articles/PMC8894894/ /pubmed/35250927 http://dx.doi.org/10.3389/fmicb.2022.805606 Text en Copyright © 2022 Hou, Wang, Ren, Meng, Yin, Zhang, Tarasiuk, Pejsak, Jiang, Mao, Zhang and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Hou, Shitong Wang, Xiangwei Ren, Shanhui Meng, Xuelian Yin, Xiangping Zhang, Jie Tarasiuk, Kazimierz Pejsak, Zygmunt Jiang, Tao Mao, Ruoqing Zhang, Yongguang Sun, Yuefeng Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication |
title | Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication |
title_full | Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication |
title_fullStr | Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication |
title_full_unstemmed | Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication |
title_short | Knockout of HDAC9 Gene Enhances Foot-and-Mouth Disease Virus Replication |
title_sort | knockout of hdac9 gene enhances foot-and-mouth disease virus replication |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894894/ https://www.ncbi.nlm.nih.gov/pubmed/35250927 http://dx.doi.org/10.3389/fmicb.2022.805606 |
work_keys_str_mv | AT houshitong knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT wangxiangwei knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT renshanhui knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT mengxuelian knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT yinxiangping knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT zhangjie knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT tarasiukkazimierz knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT pejsakzygmunt knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT jiangtao knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT maoruoqing knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT zhangyongguang knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication AT sunyuefeng knockoutofhdac9geneenhancesfootandmouthdiseasevirusreplication |