MicroED: conception, practice and future opportunities

This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today’s application to...

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Detalles Bibliográficos
Autores principales: Clabbers, Max T. B., Shiriaeva, Anna, Gonen, Tamir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Topical Reviews
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895021/
https://www.ncbi.nlm.nih.gov/pubmed/35371502
http://dx.doi.org/10.1107/S2052252521013063
Descripción
Sumario:This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today’s application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future.

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