MicroED: conception, practice and future opportunities

This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today’s application to...

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Detalles Bibliográficos
Autores principales: Clabbers, Max T. B., Shiriaeva, Anna, Gonen, Tamir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Topical Reviews
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895021/
https://www.ncbi.nlm.nih.gov/pubmed/35371502
http://dx.doi.org/10.1107/S2052252521013063
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author Clabbers, Max T. B.
Shiriaeva, Anna
Gonen, Tamir
author_facet Clabbers, Max T. B.
Shiriaeva, Anna
Gonen, Tamir
author_sort Clabbers, Max T. B.
collection PubMed
description This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today’s application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future.
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spelling pubmed-88950212022-03-31 MicroED: conception, practice and future opportunities Clabbers, Max T. B. Shiriaeva, Anna Gonen, Tamir IUCrJ Topical Reviews This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today’s application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future. International Union of Crystallography 2022-01-18 /pmc/articles/PMC8895021/ /pubmed/35371502 http://dx.doi.org/10.1107/S2052252521013063 Text en © Max T.B. Clabbers et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Topical Reviews
Clabbers, Max T. B.
Shiriaeva, Anna
Gonen, Tamir
MicroED: conception, practice and future opportunities
title MicroED: conception, practice and future opportunities
title_full MicroED: conception, practice and future opportunities
title_fullStr MicroED: conception, practice and future opportunities
title_full_unstemmed MicroED: conception, practice and future opportunities
title_short MicroED: conception, practice and future opportunities
title_sort microed: conception, practice and future opportunities
topic Topical Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895021/
https://www.ncbi.nlm.nih.gov/pubmed/35371502
http://dx.doi.org/10.1107/S2052252521013063
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