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Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue

BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a frequent and refractory disease whose pathogenesis has not yet been elucidated. Infection and other factors that reduce the local blood supply can lead to bone necrosis. AIM: To aim of this study was to assess the relationship of ONFH with bo...

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Autores principales: Liu, Chao, Li, Wei, Zhang, Chao, Pang, Feng, Wang, Da-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895187/
https://www.ncbi.nlm.nih.gov/pubmed/35321171
http://dx.doi.org/10.12998/wjcc.v10.i7.2138
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author Liu, Chao
Li, Wei
Zhang, Chao
Pang, Feng
Wang, Da-Wei
author_facet Liu, Chao
Li, Wei
Zhang, Chao
Pang, Feng
Wang, Da-Wei
author_sort Liu, Chao
collection PubMed
description BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a frequent and refractory disease whose pathogenesis has not yet been elucidated. Infection and other factors that reduce the local blood supply can lead to bone necrosis. AIM: To aim of this study was to assess the relationship of ONFH with bone infection by use of metagenomic sequencing. METHODS: Twelve patients with idiopathic ONFH and 12 comparable controls who were undergoing hip arthroplasty were followed up in parallel. Necrotic femoral head specimens were collected for bacterial and fungal cultures using standard methods. Bone specimens were subjected to preliminary processing, and metagenomics sequencing of microorganisms was performed. A one-way analysis of variance was used to compare bacterial species in the two groups. RESULTS: Bacterial and fungal cultures exhibited no evidence of microbial growth in all isolated necrotic femoral head tissues. We thus performed metagenomic sequencing and classified the species as suspected pathogens or suspected background microorganisms based on known bacterial pathogenicity. There was no evidence of viruses, fungi, parasites, M. tuberculosis complex, or mycoplasma/chlamydia. There were also no significant differences in suspected pathogens or suspected background microorganisms (P > 0.05). CONCLUSION: Although we found no pathogens specific for ONFH in necrotic femoral head tissue, our research provides a foundation for future research on the metagenomics of bone pathogens.
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spelling pubmed-88951872022-03-22 Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue Liu, Chao Li, Wei Zhang, Chao Pang, Feng Wang, Da-Wei World J Clin Cases Clinical Trials Study BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a frequent and refractory disease whose pathogenesis has not yet been elucidated. Infection and other factors that reduce the local blood supply can lead to bone necrosis. AIM: To aim of this study was to assess the relationship of ONFH with bone infection by use of metagenomic sequencing. METHODS: Twelve patients with idiopathic ONFH and 12 comparable controls who were undergoing hip arthroplasty were followed up in parallel. Necrotic femoral head specimens were collected for bacterial and fungal cultures using standard methods. Bone specimens were subjected to preliminary processing, and metagenomics sequencing of microorganisms was performed. A one-way analysis of variance was used to compare bacterial species in the two groups. RESULTS: Bacterial and fungal cultures exhibited no evidence of microbial growth in all isolated necrotic femoral head tissues. We thus performed metagenomic sequencing and classified the species as suspected pathogens or suspected background microorganisms based on known bacterial pathogenicity. There was no evidence of viruses, fungi, parasites, M. tuberculosis complex, or mycoplasma/chlamydia. There were also no significant differences in suspected pathogens or suspected background microorganisms (P > 0.05). CONCLUSION: Although we found no pathogens specific for ONFH in necrotic femoral head tissue, our research provides a foundation for future research on the metagenomics of bone pathogens. Baishideng Publishing Group Inc 2022-03-06 2022-03-06 /pmc/articles/PMC8895187/ /pubmed/35321171 http://dx.doi.org/10.12998/wjcc.v10.i7.2138 Text en ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
spellingShingle Clinical Trials Study
Liu, Chao
Li, Wei
Zhang, Chao
Pang, Feng
Wang, Da-Wei
Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue
title Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue
title_full Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue
title_fullStr Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue
title_full_unstemmed Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue
title_short Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue
title_sort previously unexplored etiology for femoral head necrosis: metagenomics detects no pathogens in necrotic femoral head tissue
topic Clinical Trials Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895187/
https://www.ncbi.nlm.nih.gov/pubmed/35321171
http://dx.doi.org/10.12998/wjcc.v10.i7.2138
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