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Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue
BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a frequent and refractory disease whose pathogenesis has not yet been elucidated. Infection and other factors that reduce the local blood supply can lead to bone necrosis. AIM: To aim of this study was to assess the relationship of ONFH with bo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895187/ https://www.ncbi.nlm.nih.gov/pubmed/35321171 http://dx.doi.org/10.12998/wjcc.v10.i7.2138 |
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author | Liu, Chao Li, Wei Zhang, Chao Pang, Feng Wang, Da-Wei |
author_facet | Liu, Chao Li, Wei Zhang, Chao Pang, Feng Wang, Da-Wei |
author_sort | Liu, Chao |
collection | PubMed |
description | BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a frequent and refractory disease whose pathogenesis has not yet been elucidated. Infection and other factors that reduce the local blood supply can lead to bone necrosis. AIM: To aim of this study was to assess the relationship of ONFH with bone infection by use of metagenomic sequencing. METHODS: Twelve patients with idiopathic ONFH and 12 comparable controls who were undergoing hip arthroplasty were followed up in parallel. Necrotic femoral head specimens were collected for bacterial and fungal cultures using standard methods. Bone specimens were subjected to preliminary processing, and metagenomics sequencing of microorganisms was performed. A one-way analysis of variance was used to compare bacterial species in the two groups. RESULTS: Bacterial and fungal cultures exhibited no evidence of microbial growth in all isolated necrotic femoral head tissues. We thus performed metagenomic sequencing and classified the species as suspected pathogens or suspected background microorganisms based on known bacterial pathogenicity. There was no evidence of viruses, fungi, parasites, M. tuberculosis complex, or mycoplasma/chlamydia. There were also no significant differences in suspected pathogens or suspected background microorganisms (P > 0.05). CONCLUSION: Although we found no pathogens specific for ONFH in necrotic femoral head tissue, our research provides a foundation for future research on the metagenomics of bone pathogens. |
format | Online Article Text |
id | pubmed-8895187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-88951872022-03-22 Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue Liu, Chao Li, Wei Zhang, Chao Pang, Feng Wang, Da-Wei World J Clin Cases Clinical Trials Study BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a frequent and refractory disease whose pathogenesis has not yet been elucidated. Infection and other factors that reduce the local blood supply can lead to bone necrosis. AIM: To aim of this study was to assess the relationship of ONFH with bone infection by use of metagenomic sequencing. METHODS: Twelve patients with idiopathic ONFH and 12 comparable controls who were undergoing hip arthroplasty were followed up in parallel. Necrotic femoral head specimens were collected for bacterial and fungal cultures using standard methods. Bone specimens were subjected to preliminary processing, and metagenomics sequencing of microorganisms was performed. A one-way analysis of variance was used to compare bacterial species in the two groups. RESULTS: Bacterial and fungal cultures exhibited no evidence of microbial growth in all isolated necrotic femoral head tissues. We thus performed metagenomic sequencing and classified the species as suspected pathogens or suspected background microorganisms based on known bacterial pathogenicity. There was no evidence of viruses, fungi, parasites, M. tuberculosis complex, or mycoplasma/chlamydia. There were also no significant differences in suspected pathogens or suspected background microorganisms (P > 0.05). CONCLUSION: Although we found no pathogens specific for ONFH in necrotic femoral head tissue, our research provides a foundation for future research on the metagenomics of bone pathogens. Baishideng Publishing Group Inc 2022-03-06 2022-03-06 /pmc/articles/PMC8895187/ /pubmed/35321171 http://dx.doi.org/10.12998/wjcc.v10.i7.2138 Text en ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
spellingShingle | Clinical Trials Study Liu, Chao Li, Wei Zhang, Chao Pang, Feng Wang, Da-Wei Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue |
title | Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue |
title_full | Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue |
title_fullStr | Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue |
title_full_unstemmed | Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue |
title_short | Previously unexplored etiology for femoral head necrosis: Metagenomics detects no pathogens in necrotic femoral head tissue |
title_sort | previously unexplored etiology for femoral head necrosis: metagenomics detects no pathogens in necrotic femoral head tissue |
topic | Clinical Trials Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895187/ https://www.ncbi.nlm.nih.gov/pubmed/35321171 http://dx.doi.org/10.12998/wjcc.v10.i7.2138 |
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