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Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells
A swab is a tool for obtaining buccal DNA from buccal mucus for biological analysis. The acquisition of a sufficient amount and high quality of DNA is an important factor in determining the accuracy of a diagnosis. A microneedle swab (MN swab) was developed to obtain more oral mucosal tissues non-in...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895340/ https://www.ncbi.nlm.nih.gov/pubmed/35252137 http://dx.doi.org/10.3389/fbioe.2022.829648 |
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author | Kim, Yun-Seo Kim, JeongHyeon Na, Woonsung Sung, Gil-Hwan Baek, Seung-Ki Kim, Yun Kyoung Kim, Gyeong Ryeong Hu, Hae-Jin Park, Jung-Hwan |
author_facet | Kim, Yun-Seo Kim, JeongHyeon Na, Woonsung Sung, Gil-Hwan Baek, Seung-Ki Kim, Yun Kyoung Kim, Gyeong Ryeong Hu, Hae-Jin Park, Jung-Hwan |
author_sort | Kim, Yun-Seo |
collection | PubMed |
description | A swab is a tool for obtaining buccal DNA from buccal mucus for biological analysis. The acquisition of a sufficient amount and high quality of DNA is an important factor in determining the accuracy of a diagnosis. A microneedle swab (MN swab) was developed to obtain more oral mucosal tissues non-invasively. Eight types of MN swabs were prepared with varying combinations of patterns (zigzag or straight), number of MNs, intervals of MNs, and sharpness of tips. When MN swab was applied up to 10 times, the tissue amount and DNA yield increased compared to commercial swabs. A zigzag pattern of microneedles was found to be more efficient than a straight pattern and increasing the number of microneedles in an array increased the DNA yield. The MN swab collected about twice the DNA compared to the commercial swab. In an in vivo test using mini pigs, the lower cycle threshold values of mucosal samples collected with MN swabs compared to samples collected with commercial swabs indicated that a greater amount of DNA was collected for SNP genotyping. A polymer MN swab is easy to manufacture by a single molding process, and it has a greater sampling capacity than existing commercial swabs. |
format | Online Article Text |
id | pubmed-8895340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88953402022-03-05 Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells Kim, Yun-Seo Kim, JeongHyeon Na, Woonsung Sung, Gil-Hwan Baek, Seung-Ki Kim, Yun Kyoung Kim, Gyeong Ryeong Hu, Hae-Jin Park, Jung-Hwan Front Bioeng Biotechnol Bioengineering and Biotechnology A swab is a tool for obtaining buccal DNA from buccal mucus for biological analysis. The acquisition of a sufficient amount and high quality of DNA is an important factor in determining the accuracy of a diagnosis. A microneedle swab (MN swab) was developed to obtain more oral mucosal tissues non-invasively. Eight types of MN swabs were prepared with varying combinations of patterns (zigzag or straight), number of MNs, intervals of MNs, and sharpness of tips. When MN swab was applied up to 10 times, the tissue amount and DNA yield increased compared to commercial swabs. A zigzag pattern of microneedles was found to be more efficient than a straight pattern and increasing the number of microneedles in an array increased the DNA yield. The MN swab collected about twice the DNA compared to the commercial swab. In an in vivo test using mini pigs, the lower cycle threshold values of mucosal samples collected with MN swabs compared to samples collected with commercial swabs indicated that a greater amount of DNA was collected for SNP genotyping. A polymer MN swab is easy to manufacture by a single molding process, and it has a greater sampling capacity than existing commercial swabs. Frontiers Media S.A. 2022-02-17 /pmc/articles/PMC8895340/ /pubmed/35252137 http://dx.doi.org/10.3389/fbioe.2022.829648 Text en Copyright © 2022 Kim, Kim, Na, Sung, Baek, Kim, Kim, Hu and Park. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Kim, Yun-Seo Kim, JeongHyeon Na, Woonsung Sung, Gil-Hwan Baek, Seung-Ki Kim, Yun Kyoung Kim, Gyeong Ryeong Hu, Hae-Jin Park, Jung-Hwan Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells |
title | Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells |
title_full | Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells |
title_fullStr | Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells |
title_full_unstemmed | Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells |
title_short | Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells |
title_sort | development of a microneedle swab for acquisition of genomic dna from buccal cells |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895340/ https://www.ncbi.nlm.nih.gov/pubmed/35252137 http://dx.doi.org/10.3389/fbioe.2022.829648 |
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