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Histone H2B Deacylation Selectivity: Exploring Chromatin’s Dark Matter with an Engineered Sortase
[Image: see text] We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and β-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895396/ https://www.ncbi.nlm.nih.gov/pubmed/35175758 http://dx.doi.org/10.1021/jacs.1c13555 |
| _version_ | 1784662916604100608 |
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| author | Wang, Zhipeng A. Whedon, Samuel D. Wu, Mingxuan Wang, Siyu Brown, Edward A. Anmangandla, Ananya Regan, Liam Lee, Kwangwoon Du, Jianfeng Hong, Jun Young Fairall, Louise Kay, Taylor Lin, Hening Zhao, Yingming Schwabe, John W. R. Cole, Philip A. |
| author_facet | Wang, Zhipeng A. Whedon, Samuel D. Wu, Mingxuan Wang, Siyu Brown, Edward A. Anmangandla, Ananya Regan, Liam Lee, Kwangwoon Du, Jianfeng Hong, Jun Young Fairall, Louise Kay, Taylor Lin, Hening Zhao, Yingming Schwabe, John W. R. Cole, Philip A. |
| author_sort | Wang, Zhipeng A. |
| collection | PubMed |
| description | [Image: see text] We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and β-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics. |
| format | Online Article Text |
| id | pubmed-8895396 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | American Chemical Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-88953962022-03-07 Histone H2B Deacylation Selectivity: Exploring Chromatin’s Dark Matter with an Engineered Sortase Wang, Zhipeng A. Whedon, Samuel D. Wu, Mingxuan Wang, Siyu Brown, Edward A. Anmangandla, Ananya Regan, Liam Lee, Kwangwoon Du, Jianfeng Hong, Jun Young Fairall, Louise Kay, Taylor Lin, Hening Zhao, Yingming Schwabe, John W. R. Cole, Philip A. J Am Chem Soc [Image: see text] We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and β-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics. American Chemical Society 2022-02-17 2022-03-02 /pmc/articles/PMC8895396/ /pubmed/35175758 http://dx.doi.org/10.1021/jacs.1c13555 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Wang, Zhipeng A. Whedon, Samuel D. Wu, Mingxuan Wang, Siyu Brown, Edward A. Anmangandla, Ananya Regan, Liam Lee, Kwangwoon Du, Jianfeng Hong, Jun Young Fairall, Louise Kay, Taylor Lin, Hening Zhao, Yingming Schwabe, John W. R. Cole, Philip A. Histone H2B Deacylation Selectivity: Exploring Chromatin’s Dark Matter with an Engineered Sortase |
| title | Histone
H2B Deacylation Selectivity: Exploring Chromatin’s
Dark Matter with an Engineered Sortase |
| title_full | Histone
H2B Deacylation Selectivity: Exploring Chromatin’s
Dark Matter with an Engineered Sortase |
| title_fullStr | Histone
H2B Deacylation Selectivity: Exploring Chromatin’s
Dark Matter with an Engineered Sortase |
| title_full_unstemmed | Histone
H2B Deacylation Selectivity: Exploring Chromatin’s
Dark Matter with an Engineered Sortase |
| title_short | Histone
H2B Deacylation Selectivity: Exploring Chromatin’s
Dark Matter with an Engineered Sortase |
| title_sort | histone
h2b deacylation selectivity: exploring chromatin’s
dark matter with an engineered sortase |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895396/ https://www.ncbi.nlm.nih.gov/pubmed/35175758 http://dx.doi.org/10.1021/jacs.1c13555 |
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