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Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits

BACKGROUND: To date, the usefulness of parathyroid hormone [PTH (1–34)] in distraction osteogenesis has been reported in several studies. We aimed to determine the optimal timing of PTH (1–34) administration in a rabbit distraction osteogenesis model. METHODS: The lower hind leg of a Japanese white...

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Autores principales: Inada, Narisaku, Ohata, Tetsuya, Maruno, Hideto, Morii, Takeshi, Hosogane, Naobumi, Ichimura, Shoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895655/
https://www.ncbi.nlm.nih.gov/pubmed/35241115
http://dx.doi.org/10.1186/s13018-022-03019-2
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author Inada, Narisaku
Ohata, Tetsuya
Maruno, Hideto
Morii, Takeshi
Hosogane, Naobumi
Ichimura, Shoichi
author_facet Inada, Narisaku
Ohata, Tetsuya
Maruno, Hideto
Morii, Takeshi
Hosogane, Naobumi
Ichimura, Shoichi
author_sort Inada, Narisaku
collection PubMed
description BACKGROUND: To date, the usefulness of parathyroid hormone [PTH (1–34)] in distraction osteogenesis has been reported in several studies. We aimed to determine the optimal timing of PTH (1–34) administration in a rabbit distraction osteogenesis model. METHODS: The lower hind leg of a Japanese white rabbit was externally fixed, and tibial osteotomy was performed. One week after the osteotomy, bone lengthening was carried out at 0.375 mm/12 h for 2 weeks. After 5 weeks, the lower leg bone was collected. Bone mineral density (BMD), peripheral quantitative computed tomography (pQCT), micro-computed tomography (micro-CT), and mechanical tests were performed on the distracted callus. The rabbits were divided into three groups according to the timing of PTH (1–34) administration: 4 weeks during the distraction and consolidation phases (group D + C), 2 weeks of the distraction phase (group D), and the first 2 weeks of the consolidation phase (group C). A control group (group N) was administered saline for 4 weeks during the distraction and consolidation phases. Furthermore, to obtain histological findings, lower leg bones were collected from each rabbit at 2, 3, and 4 weeks after osteotomy, and tissue sections of the distracted callus were examined histologically. RESULTS: The BMD was highest in group C and was significantly higher than group D. In pQCT, the total cross-sectional area was significantly higher in groups D + C, D, and C than group N, and the cortical bone area was highest in group C and was significantly higher than group D. In micro-CT, group C had the highest bone mass and number of trabeculae. Regarding the mechanical test, group C had the highest callus failure strength, and this value was significantly higher compared to group N. There was no significant difference between groups D and N. The histological findings revealed that the distracted callus mainly consisted of endochondral ossification in the distraction phase. In the consolidation phase, the chondrocytes were almost absent, and intramembranous ossification was the main type of ossification. CONCLUSION: We found that the optimal timing of PTH (1–34) administration is during the consolidation phase, which is mainly characterized by intramembranous ossification.
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spelling pubmed-88956552022-03-10 Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits Inada, Narisaku Ohata, Tetsuya Maruno, Hideto Morii, Takeshi Hosogane, Naobumi Ichimura, Shoichi J Orthop Surg Res Research Article BACKGROUND: To date, the usefulness of parathyroid hormone [PTH (1–34)] in distraction osteogenesis has been reported in several studies. We aimed to determine the optimal timing of PTH (1–34) administration in a rabbit distraction osteogenesis model. METHODS: The lower hind leg of a Japanese white rabbit was externally fixed, and tibial osteotomy was performed. One week after the osteotomy, bone lengthening was carried out at 0.375 mm/12 h for 2 weeks. After 5 weeks, the lower leg bone was collected. Bone mineral density (BMD), peripheral quantitative computed tomography (pQCT), micro-computed tomography (micro-CT), and mechanical tests were performed on the distracted callus. The rabbits were divided into three groups according to the timing of PTH (1–34) administration: 4 weeks during the distraction and consolidation phases (group D + C), 2 weeks of the distraction phase (group D), and the first 2 weeks of the consolidation phase (group C). A control group (group N) was administered saline for 4 weeks during the distraction and consolidation phases. Furthermore, to obtain histological findings, lower leg bones were collected from each rabbit at 2, 3, and 4 weeks after osteotomy, and tissue sections of the distracted callus were examined histologically. RESULTS: The BMD was highest in group C and was significantly higher than group D. In pQCT, the total cross-sectional area was significantly higher in groups D + C, D, and C than group N, and the cortical bone area was highest in group C and was significantly higher than group D. In micro-CT, group C had the highest bone mass and number of trabeculae. Regarding the mechanical test, group C had the highest callus failure strength, and this value was significantly higher compared to group N. There was no significant difference between groups D and N. The histological findings revealed that the distracted callus mainly consisted of endochondral ossification in the distraction phase. In the consolidation phase, the chondrocytes were almost absent, and intramembranous ossification was the main type of ossification. CONCLUSION: We found that the optimal timing of PTH (1–34) administration is during the consolidation phase, which is mainly characterized by intramembranous ossification. BioMed Central 2022-03-03 /pmc/articles/PMC8895655/ /pubmed/35241115 http://dx.doi.org/10.1186/s13018-022-03019-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Inada, Narisaku
Ohata, Tetsuya
Maruno, Hideto
Morii, Takeshi
Hosogane, Naobumi
Ichimura, Shoichi
Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits
title Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits
title_full Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits
title_fullStr Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits
title_full_unstemmed Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits
title_short Optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits
title_sort optimal timing for intermittent administration of parathyroid hormone (1–34) for distraction osteogenesis in rabbits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895655/
https://www.ncbi.nlm.nih.gov/pubmed/35241115
http://dx.doi.org/10.1186/s13018-022-03019-2
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