Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases

Epstein–Barr virus DNA viral load is used as a surrogate marker to start Rituximab in transplant recipients at risk of developing PTLD. However, an elevated EBV DNAemia does not discriminate lymphoproliferation and replication. We designed a new molecular assay (methyl-qPCR) to distinguish methylate...

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Autores principales: Borde, Chloé, Quignon, Frédérique, Amiel, Corinne, Gozlan, Joël, Marechal, Vincent, Brissot, Eolia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895795/
https://www.ncbi.nlm.nih.gov/pubmed/35246247
http://dx.doi.org/10.1186/s13148-022-01255-1
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author Borde, Chloé
Quignon, Frédérique
Amiel, Corinne
Gozlan, Joël
Marechal, Vincent
Brissot, Eolia
author_facet Borde, Chloé
Quignon, Frédérique
Amiel, Corinne
Gozlan, Joël
Marechal, Vincent
Brissot, Eolia
author_sort Borde, Chloé
collection PubMed
description Epstein–Barr virus DNA viral load is used as a surrogate marker to start Rituximab in transplant recipients at risk of developing PTLD. However, an elevated EBV DNAemia does not discriminate lymphoproliferation and replication. We designed a new molecular assay (methyl-qPCR) to distinguish methylated versus unmethylated viral genomes. In blood, viral genomes were highly methylated in EBV primary infections, PTLD and 4/5 transplant recipients with high viral load. The only patient with under-methylated EBV genomes did not respond to rituximab. Methyl-qPCR is a convenient method to discriminate between latent and lytic EBV genomes and could be useful in treatment decisions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-022-01255-1.
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spelling pubmed-88957952022-03-10 Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases Borde, Chloé Quignon, Frédérique Amiel, Corinne Gozlan, Joël Marechal, Vincent Brissot, Eolia Clin Epigenetics Short Report Epstein–Barr virus DNA viral load is used as a surrogate marker to start Rituximab in transplant recipients at risk of developing PTLD. However, an elevated EBV DNAemia does not discriminate lymphoproliferation and replication. We designed a new molecular assay (methyl-qPCR) to distinguish methylated versus unmethylated viral genomes. In blood, viral genomes were highly methylated in EBV primary infections, PTLD and 4/5 transplant recipients with high viral load. The only patient with under-methylated EBV genomes did not respond to rituximab. Methyl-qPCR is a convenient method to discriminate between latent and lytic EBV genomes and could be useful in treatment decisions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-022-01255-1. BioMed Central 2022-03-04 /pmc/articles/PMC8895795/ /pubmed/35246247 http://dx.doi.org/10.1186/s13148-022-01255-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Borde, Chloé
Quignon, Frédérique
Amiel, Corinne
Gozlan, Joël
Marechal, Vincent
Brissot, Eolia
Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases
title Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases
title_full Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases
title_fullStr Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases
title_full_unstemmed Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases
title_short Methyl-qPCR: a new method to investigate Epstein–Barr virus infection in post-transplant lymphoproliferative diseases
title_sort methyl-qpcr: a new method to investigate epstein–barr virus infection in post-transplant lymphoproliferative diseases
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895795/
https://www.ncbi.nlm.nih.gov/pubmed/35246247
http://dx.doi.org/10.1186/s13148-022-01255-1
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