Cargando…

A conditional glutamatergic synaptic vesicle marker for Drosophila

Glutamate is a principal neurotransmitter used extensively by the nervous systems of all vertebrate and invertebrate animals. It is primarily an excitatory neurotransmitter that has been implicated in nervous system development, as well as a myriad of brain functions from the simple transmission of...

Descripción completa

Detalles Bibliográficos
Autores principales: Certel, Sarah J, Ruchti, Evelyne, McCabe, Brian D, Stowers, R Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895992/
https://www.ncbi.nlm.nih.gov/pubmed/35100385
http://dx.doi.org/10.1093/g3journal/jkab453
_version_ 1784663048610381824
author Certel, Sarah J
Ruchti, Evelyne
McCabe, Brian D
Stowers, R Steven
author_facet Certel, Sarah J
Ruchti, Evelyne
McCabe, Brian D
Stowers, R Steven
author_sort Certel, Sarah J
collection PubMed
description Glutamate is a principal neurotransmitter used extensively by the nervous systems of all vertebrate and invertebrate animals. It is primarily an excitatory neurotransmitter that has been implicated in nervous system development, as well as a myriad of brain functions from the simple transmission of information between neurons to more complex aspects of nervous system function including synaptic plasticity, learning, and memory. Identification of glutamatergic neurons and their sites of glutamate release are thus essential for understanding the mechanisms of neural circuit function and how information is processed to generate behavior. Here, we describe and characterize smFLAG-vGlut, a conditional marker of glutamatergic synaptic vesicles for the Drosophila model system. smFLAG-vGlut is validated for functionality, conditional expression, and specificity for glutamatergic neurons and synaptic vesicles. The utility of smFLAG-vGlut is demonstrated by glutamatergic neurotransmitter phenotyping of 26 different central complex neuron types of which nine were established to be glutamatergic. This illumination of glutamate neurotransmitter usage will enhance the modeling of central complex neural circuitry and thereby our understanding of information processing by this region of the fly brain. The use of smFLAG for glutamatergic neurotransmitter phenotyping and identification of glutamate release sites can be extended to any Drosophila neuron(s) represented by a binary transcription system driver.
format Online
Article
Text
id pubmed-8895992
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-88959922022-03-07 A conditional glutamatergic synaptic vesicle marker for Drosophila Certel, Sarah J Ruchti, Evelyne McCabe, Brian D Stowers, R Steven G3 (Bethesda) Investigation Glutamate is a principal neurotransmitter used extensively by the nervous systems of all vertebrate and invertebrate animals. It is primarily an excitatory neurotransmitter that has been implicated in nervous system development, as well as a myriad of brain functions from the simple transmission of information between neurons to more complex aspects of nervous system function including synaptic plasticity, learning, and memory. Identification of glutamatergic neurons and their sites of glutamate release are thus essential for understanding the mechanisms of neural circuit function and how information is processed to generate behavior. Here, we describe and characterize smFLAG-vGlut, a conditional marker of glutamatergic synaptic vesicles for the Drosophila model system. smFLAG-vGlut is validated for functionality, conditional expression, and specificity for glutamatergic neurons and synaptic vesicles. The utility of smFLAG-vGlut is demonstrated by glutamatergic neurotransmitter phenotyping of 26 different central complex neuron types of which nine were established to be glutamatergic. This illumination of glutamate neurotransmitter usage will enhance the modeling of central complex neural circuitry and thereby our understanding of information processing by this region of the fly brain. The use of smFLAG for glutamatergic neurotransmitter phenotyping and identification of glutamate release sites can be extended to any Drosophila neuron(s) represented by a binary transcription system driver. Oxford University Press 2022-01-03 /pmc/articles/PMC8895992/ /pubmed/35100385 http://dx.doi.org/10.1093/g3journal/jkab453 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Certel, Sarah J
Ruchti, Evelyne
McCabe, Brian D
Stowers, R Steven
A conditional glutamatergic synaptic vesicle marker for Drosophila
title A conditional glutamatergic synaptic vesicle marker for Drosophila
title_full A conditional glutamatergic synaptic vesicle marker for Drosophila
title_fullStr A conditional glutamatergic synaptic vesicle marker for Drosophila
title_full_unstemmed A conditional glutamatergic synaptic vesicle marker for Drosophila
title_short A conditional glutamatergic synaptic vesicle marker for Drosophila
title_sort conditional glutamatergic synaptic vesicle marker for drosophila
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895992/
https://www.ncbi.nlm.nih.gov/pubmed/35100385
http://dx.doi.org/10.1093/g3journal/jkab453
work_keys_str_mv AT certelsarahj aconditionalglutamatergicsynapticvesiclemarkerfordrosophila
AT ruchtievelyne aconditionalglutamatergicsynapticvesiclemarkerfordrosophila
AT mccabebriand aconditionalglutamatergicsynapticvesiclemarkerfordrosophila
AT stowersrsteven aconditionalglutamatergicsynapticvesiclemarkerfordrosophila
AT certelsarahj conditionalglutamatergicsynapticvesiclemarkerfordrosophila
AT ruchtievelyne conditionalglutamatergicsynapticvesiclemarkerfordrosophila
AT mccabebriand conditionalglutamatergicsynapticvesiclemarkerfordrosophila
AT stowersrsteven conditionalglutamatergicsynapticvesiclemarkerfordrosophila