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In vitro and in vivo anti-Candida activity of citral in combination with fluconazole

BACKGROUND: The ability of Candida to develop biofilms on inert surfaces or living tissues favors recalcitrant and chronic candidiasis associated, in many instances, with resistance to current antifungal therapy. AIM: The aim of this study was to evaluate the antifungal activity of citral, a phytoco...

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Autores principales: Miranda-Cadena, Katherine, Marcos-Arias, Cristina, Perez-Rodriguez, Aitzol, Cabello-Beitia, Iván, Mateo, Estibaliz, Sevillano, Elena, Madariaga, Lucila, Quindós, Guillermo, Eraso, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896188/
https://www.ncbi.nlm.nih.gov/pubmed/35251524
http://dx.doi.org/10.1080/20002297.2022.2045813
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author Miranda-Cadena, Katherine
Marcos-Arias, Cristina
Perez-Rodriguez, Aitzol
Cabello-Beitia, Iván
Mateo, Estibaliz
Sevillano, Elena
Madariaga, Lucila
Quindós, Guillermo
Eraso, Elena
author_facet Miranda-Cadena, Katherine
Marcos-Arias, Cristina
Perez-Rodriguez, Aitzol
Cabello-Beitia, Iván
Mateo, Estibaliz
Sevillano, Elena
Madariaga, Lucila
Quindós, Guillermo
Eraso, Elena
author_sort Miranda-Cadena, Katherine
collection PubMed
description BACKGROUND: The ability of Candida to develop biofilms on inert surfaces or living tissues favors recalcitrant and chronic candidiasis associated, in many instances, with resistance to current antifungal therapy. AIM: The aim of this study was to evaluate the antifungal activity of citral, a phytocompound present in lemongrass essential oil, in monotherapy and combined with fluconazole against azole-resistant Candida planktonic cells and biofilms. The effect of citral combined with fluconazole was also analysed with regard to the expression of fluconazole resistance-associated genes in Candida albicans and the effectiveness of the combination therapy in a Caenorhabditis elegans model of candidiasis. RESULTS: Citral reduced biofilm formation at initial stages and the metabolic activity of the mature biofilm. The combination of citral with fluconazole was synergistic, with a significant increase in the survival of C. elegans infected with Candida. RNA analysis revealed a reduction of the expression of the efflux pump encoded by MDR1, leading to a greater effect of fluconazole. CONCLUSION: Citral in monotherapy and in combination with fluconazole could represent an interesting therapy to treat recalcitrant Candida infections associated to biofilms.
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spelling pubmed-88961882022-03-05 In vitro and in vivo anti-Candida activity of citral in combination with fluconazole Miranda-Cadena, Katherine Marcos-Arias, Cristina Perez-Rodriguez, Aitzol Cabello-Beitia, Iván Mateo, Estibaliz Sevillano, Elena Madariaga, Lucila Quindós, Guillermo Eraso, Elena J Oral Microbiol Original Article BACKGROUND: The ability of Candida to develop biofilms on inert surfaces or living tissues favors recalcitrant and chronic candidiasis associated, in many instances, with resistance to current antifungal therapy. AIM: The aim of this study was to evaluate the antifungal activity of citral, a phytocompound present in lemongrass essential oil, in monotherapy and combined with fluconazole against azole-resistant Candida planktonic cells and biofilms. The effect of citral combined with fluconazole was also analysed with regard to the expression of fluconazole resistance-associated genes in Candida albicans and the effectiveness of the combination therapy in a Caenorhabditis elegans model of candidiasis. RESULTS: Citral reduced biofilm formation at initial stages and the metabolic activity of the mature biofilm. The combination of citral with fluconazole was synergistic, with a significant increase in the survival of C. elegans infected with Candida. RNA analysis revealed a reduction of the expression of the efflux pump encoded by MDR1, leading to a greater effect of fluconazole. CONCLUSION: Citral in monotherapy and in combination with fluconazole could represent an interesting therapy to treat recalcitrant Candida infections associated to biofilms. Taylor & Francis 2022-03-02 /pmc/articles/PMC8896188/ /pubmed/35251524 http://dx.doi.org/10.1080/20002297.2022.2045813 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Miranda-Cadena, Katherine
Marcos-Arias, Cristina
Perez-Rodriguez, Aitzol
Cabello-Beitia, Iván
Mateo, Estibaliz
Sevillano, Elena
Madariaga, Lucila
Quindós, Guillermo
Eraso, Elena
In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_full In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_fullStr In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_full_unstemmed In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_short In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
title_sort in vitro and in vivo anti-candida activity of citral in combination with fluconazole
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896188/
https://www.ncbi.nlm.nih.gov/pubmed/35251524
http://dx.doi.org/10.1080/20002297.2022.2045813
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