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Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China

Colistin is regarded as a last-resort agent to combat infections caused by multidrug-resistant (MDR) Gram-negative bacteria, especially carbapenem-resistant isolates. In recent years, reports of colistin-resistant Klebsiella pneumoniae (CoRKp) are increasing. However, the molecular mechanism and rel...

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Autores principales: Liu, Xiaoyu, Wu, Yarong, Zhu, Ying, Jia, Peiyao, Li, Xue, Jia, Xinmiao, Yu, Wei, Cui, Yujun, Yang, Ruifu, Xia, Wei, Xu, Yingchun, Yang, Qiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896207/
https://www.ncbi.nlm.nih.gov/pubmed/35086435
http://dx.doi.org/10.1080/22221751.2022.2036078
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author Liu, Xiaoyu
Wu, Yarong
Zhu, Ying
Jia, Peiyao
Li, Xue
Jia, Xinmiao
Yu, Wei
Cui, Yujun
Yang, Ruifu
Xia, Wei
Xu, Yingchun
Yang, Qiwen
author_facet Liu, Xiaoyu
Wu, Yarong
Zhu, Ying
Jia, Peiyao
Li, Xue
Jia, Xinmiao
Yu, Wei
Cui, Yujun
Yang, Ruifu
Xia, Wei
Xu, Yingchun
Yang, Qiwen
author_sort Liu, Xiaoyu
collection PubMed
description Colistin is regarded as a last-resort agent to combat infections caused by multidrug-resistant (MDR) Gram-negative bacteria, especially carbapenem-resistant isolates. In recent years, reports of colistin-resistant Klebsiella pneumoniae (CoRKp) are increasing. However, the molecular mechanism and relevance of colistin resistance and virulence remain unclear. Fourteen CoRKp strains were retrospectively screened from 1884 clinical K. pneumoniae isolates during 2017–2018 in China. Six CoRKp strains belonging to ST11 were MDR strains. Plasmid-mediated mobile colistin-resistance genes had a low prevalence in CoRKp. Our results revealed that up-regulated expression of two-component systems, especially phoPQ, contributed more to colistin resistance. mgrB mutation was the most common molecular mechanism of colistin resistance, caused by either nonsense mutations or insertion sequences, which drove the overexpression of phoPQ system. This study also identified three novel point mutations in pmrAB system, in which D313N mutation in pmrB was proved to increase the MIC to colistin by 16-fold. In addition, 6 out of 14 CoRKP strains independently carried hypervirulence genes. All six strains showed medium-to-high virulence phenotype compared with NTUH-K2044 strain in mice intraperitoneal challenge models. We found that 4 strains were biofilm strong producers and transcriptome analysis revealed that three of them significantly up-regulated expression of type III fimbrial shaft gene mrkA. In conclusion, our result revealed the emergence of colistin-resistant and hypervirulent MDR K. pneumoniae, which is a noticeable superbug and could cause a severe challenge to public health.
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spelling pubmed-88962072022-03-05 Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China Liu, Xiaoyu Wu, Yarong Zhu, Ying Jia, Peiyao Li, Xue Jia, Xinmiao Yu, Wei Cui, Yujun Yang, Ruifu Xia, Wei Xu, Yingchun Yang, Qiwen Emerg Microbes Infect Antimicrobial Agents Colistin is regarded as a last-resort agent to combat infections caused by multidrug-resistant (MDR) Gram-negative bacteria, especially carbapenem-resistant isolates. In recent years, reports of colistin-resistant Klebsiella pneumoniae (CoRKp) are increasing. However, the molecular mechanism and relevance of colistin resistance and virulence remain unclear. Fourteen CoRKp strains were retrospectively screened from 1884 clinical K. pneumoniae isolates during 2017–2018 in China. Six CoRKp strains belonging to ST11 were MDR strains. Plasmid-mediated mobile colistin-resistance genes had a low prevalence in CoRKp. Our results revealed that up-regulated expression of two-component systems, especially phoPQ, contributed more to colistin resistance. mgrB mutation was the most common molecular mechanism of colistin resistance, caused by either nonsense mutations or insertion sequences, which drove the overexpression of phoPQ system. This study also identified three novel point mutations in pmrAB system, in which D313N mutation in pmrB was proved to increase the MIC to colistin by 16-fold. In addition, 6 out of 14 CoRKP strains independently carried hypervirulence genes. All six strains showed medium-to-high virulence phenotype compared with NTUH-K2044 strain in mice intraperitoneal challenge models. We found that 4 strains were biofilm strong producers and transcriptome analysis revealed that three of them significantly up-regulated expression of type III fimbrial shaft gene mrkA. In conclusion, our result revealed the emergence of colistin-resistant and hypervirulent MDR K. pneumoniae, which is a noticeable superbug and could cause a severe challenge to public health. Taylor & Francis 2022-03-03 /pmc/articles/PMC8896207/ /pubmed/35086435 http://dx.doi.org/10.1080/22221751.2022.2036078 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Antimicrobial Agents
Liu, Xiaoyu
Wu, Yarong
Zhu, Ying
Jia, Peiyao
Li, Xue
Jia, Xinmiao
Yu, Wei
Cui, Yujun
Yang, Ruifu
Xia, Wei
Xu, Yingchun
Yang, Qiwen
Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China
title Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China
title_full Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China
title_fullStr Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China
title_full_unstemmed Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China
title_short Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China
title_sort emergence of colistin-resistant hypervirulent klebsiella pneumoniae (cor-hvkp) in china
topic Antimicrobial Agents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896207/
https://www.ncbi.nlm.nih.gov/pubmed/35086435
http://dx.doi.org/10.1080/22221751.2022.2036078
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