CRAM 3.1: advances in the CRAM file format

MOTIVATION: CRAM has established itself as a high compression alternative to the BAM file format for DNA sequencing data. We describe updates to further improve this on modern sequencing instruments. RESULTS: With Illumina data CRAM 3.1 is 7–15% smaller than the equivalent CRAM 3.0 file, and 50–70%...

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Detalles Bibliográficos
Autor principal: Bonfield, James K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Original Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896640/
https://www.ncbi.nlm.nih.gov/pubmed/34999766
http://dx.doi.org/10.1093/bioinformatics/btac010
Descripción
Sumario:MOTIVATION: CRAM has established itself as a high compression alternative to the BAM file format for DNA sequencing data. We describe updates to further improve this on modern sequencing instruments. RESULTS: With Illumina data CRAM 3.1 is 7–15% smaller than the equivalent CRAM 3.0 file, and 50–70% smaller than the corresponding BAM file. Long-read technology shows more modest compression due to the presence of high-entropy signals. AVAILABILITY AND IMPLEMENTATION: The CRAM 3.0 specification is freely available from https://samtools.github.io/hts-specs/CRAMv3.pdf. The CRAM 3.1 improvements are available in a separate OpenSource HTScodecs library from https://github.com/samtools/htscodecs, and have been incorporated into HTSlib. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

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