Cargando…

Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs

Polymeric immunoglobulin receptor (pIgR), the transmembrane transporter of polymeric immunoglobulin A and M, has multiple immune functions. To explore the characteristics of pIgR expression in Bactrian camel lungs, twelve healthy adult (2–7 years old) Bactrian camels were systematically studied. The...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Wan-hong, Zhang, Wang-dong, Cheng, Cui-cui, Lu, Jia, Liu, Lei, Chen, Zhi-hua, Wang, Wen-hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896721/
https://www.ncbi.nlm.nih.gov/pubmed/35245335
http://dx.doi.org/10.1371/journal.pone.0264815
_version_ 1784663225791414272
author He, Wan-hong
Zhang, Wang-dong
Cheng, Cui-cui
Lu, Jia
Liu, Lei
Chen, Zhi-hua
Wang, Wen-hui
author_facet He, Wan-hong
Zhang, Wang-dong
Cheng, Cui-cui
Lu, Jia
Liu, Lei
Chen, Zhi-hua
Wang, Wen-hui
author_sort He, Wan-hong
collection PubMed
description Polymeric immunoglobulin receptor (pIgR), the transmembrane transporter of polymeric immunoglobulin A and M, has multiple immune functions. To explore the characteristics of pIgR expression in Bactrian camel lungs, twelve healthy adult (2–7 years old) Bactrian camels were systematically studied. The results showed that pIgR was mainly expressed in the cytoplasm and membrane of ciliated cells, as well as in the cytoplasm and membrane of basal cells, serous cells of bronchial glands, club cells and alveolar type 2 cells in Bactrian camel lungs. Specially, as the bronchial branches extended, the pIgR expression level in ciliated cells significantly declined (p<0.05), and the corresponding bronchial luminal areas obviously decreased (p<0.05). However, pIgR was not expressed in goblet cells, endocrine cells, alveolar type 1 cells and mucous cells of bronchial glands. The results demonstrated that ciliated cells continuously distributed throughout the whole bronchial tree mucosa were the major expression sites of pIgR, and pIgR was also expressed in basal cells, serous cells of bronchial glands, club cells and alveolar type 2 cells, which would facilitate secretory immunoglobulin A (SIgA) transmembrane transport by pIgR and form an intact protective barrier. Moreover, the pIgR expression level in ciliated cells was positively correlated with the bronchial luminal areas; but negatively correlated with the cleanliness of airflow through the bronchial cross-sections, showing that the pIgR expression level in the bronchial epithelium was inhomogeneous. Our study provided a foundation for further exploring the regulatory functions of immunoglobulins (i.e., SIgA) after transport across the membrane by pIgR in Bactrian camel lungs.
format Online
Article
Text
id pubmed-8896721
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-88967212022-03-05 Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs He, Wan-hong Zhang, Wang-dong Cheng, Cui-cui Lu, Jia Liu, Lei Chen, Zhi-hua Wang, Wen-hui PLoS One Research Article Polymeric immunoglobulin receptor (pIgR), the transmembrane transporter of polymeric immunoglobulin A and M, has multiple immune functions. To explore the characteristics of pIgR expression in Bactrian camel lungs, twelve healthy adult (2–7 years old) Bactrian camels were systematically studied. The results showed that pIgR was mainly expressed in the cytoplasm and membrane of ciliated cells, as well as in the cytoplasm and membrane of basal cells, serous cells of bronchial glands, club cells and alveolar type 2 cells in Bactrian camel lungs. Specially, as the bronchial branches extended, the pIgR expression level in ciliated cells significantly declined (p<0.05), and the corresponding bronchial luminal areas obviously decreased (p<0.05). However, pIgR was not expressed in goblet cells, endocrine cells, alveolar type 1 cells and mucous cells of bronchial glands. The results demonstrated that ciliated cells continuously distributed throughout the whole bronchial tree mucosa were the major expression sites of pIgR, and pIgR was also expressed in basal cells, serous cells of bronchial glands, club cells and alveolar type 2 cells, which would facilitate secretory immunoglobulin A (SIgA) transmembrane transport by pIgR and form an intact protective barrier. Moreover, the pIgR expression level in ciliated cells was positively correlated with the bronchial luminal areas; but negatively correlated with the cleanliness of airflow through the bronchial cross-sections, showing that the pIgR expression level in the bronchial epithelium was inhomogeneous. Our study provided a foundation for further exploring the regulatory functions of immunoglobulins (i.e., SIgA) after transport across the membrane by pIgR in Bactrian camel lungs. Public Library of Science 2022-03-04 /pmc/articles/PMC8896721/ /pubmed/35245335 http://dx.doi.org/10.1371/journal.pone.0264815 Text en © 2022 He et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
He, Wan-hong
Zhang, Wang-dong
Cheng, Cui-cui
Lu, Jia
Liu, Lei
Chen, Zhi-hua
Wang, Wen-hui
Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs
title Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs
title_full Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs
title_fullStr Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs
title_full_unstemmed Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs
title_short Expression characteristics of polymeric immunoglobulin receptor in Bactrian camel (Camelus bactrianus) lungs
title_sort expression characteristics of polymeric immunoglobulin receptor in bactrian camel (camelus bactrianus) lungs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896721/
https://www.ncbi.nlm.nih.gov/pubmed/35245335
http://dx.doi.org/10.1371/journal.pone.0264815
work_keys_str_mv AT hewanhong expressioncharacteristicsofpolymericimmunoglobulinreceptorinbactriancamelcamelusbactrianuslungs
AT zhangwangdong expressioncharacteristicsofpolymericimmunoglobulinreceptorinbactriancamelcamelusbactrianuslungs
AT chengcuicui expressioncharacteristicsofpolymericimmunoglobulinreceptorinbactriancamelcamelusbactrianuslungs
AT lujia expressioncharacteristicsofpolymericimmunoglobulinreceptorinbactriancamelcamelusbactrianuslungs
AT liulei expressioncharacteristicsofpolymericimmunoglobulinreceptorinbactriancamelcamelusbactrianuslungs
AT chenzhihua expressioncharacteristicsofpolymericimmunoglobulinreceptorinbactriancamelcamelusbactrianuslungs
AT wangwenhui expressioncharacteristicsofpolymericimmunoglobulinreceptorinbactriancamelcamelusbactrianuslungs