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Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe

Imaging chromatin organization at the molecular-scale resolution remains an important endeavor in basic and translational research. Stochastic optical reconstruction microscopy (STORM) is a powerful superresolution imaging technique to visualize nanoscale molecular organization down to the resolutio...

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Autores principales: Xu, Jianquan, Sun, Xuejiao, Kim, Kwangho, Brand, Rhonda M., Hartman, Douglas, Ma, Hongqiang, Brand, Randall E., Bai, Mingfeng, Liu, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896800/
https://www.ncbi.nlm.nih.gov/pubmed/35245126
http://dx.doi.org/10.1126/sciadv.abm8293
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author Xu, Jianquan
Sun, Xuejiao
Kim, Kwangho
Brand, Rhonda M.
Hartman, Douglas
Ma, Hongqiang
Brand, Randall E.
Bai, Mingfeng
Liu, Yang
author_facet Xu, Jianquan
Sun, Xuejiao
Kim, Kwangho
Brand, Rhonda M.
Hartman, Douglas
Ma, Hongqiang
Brand, Randall E.
Bai, Mingfeng
Liu, Yang
author_sort Xu, Jianquan
collection PubMed
description Imaging chromatin organization at the molecular-scale resolution remains an important endeavor in basic and translational research. Stochastic optical reconstruction microscopy (STORM) is a powerful superresolution imaging technique to visualize nanoscale molecular organization down to the resolution of ~20 to 30 nm. Despite the substantial progress in imaging chromatin organization in cells and model systems, its routine application on assessing pathological tissue remains limited. It is, in part, hampered by the lack of simple labels that consistently generates high-quality STORM images on the highly processed clinical tissue. We developed a fast, simple, and robust small-molecule fluorescent probe—cyanine 5–conjugated Hoechst—for routine superresolution imaging of nanoscale nuclear architecture on clinical tissue. We demonstrated the biological and clinical significance of imaging superresolved chromatin structure in cancer development and its potential clinical utility for cancer risk stratification.
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spelling pubmed-88968002022-03-14 Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe Xu, Jianquan Sun, Xuejiao Kim, Kwangho Brand, Rhonda M. Hartman, Douglas Ma, Hongqiang Brand, Randall E. Bai, Mingfeng Liu, Yang Sci Adv Biomedicine and Life Sciences Imaging chromatin organization at the molecular-scale resolution remains an important endeavor in basic and translational research. Stochastic optical reconstruction microscopy (STORM) is a powerful superresolution imaging technique to visualize nanoscale molecular organization down to the resolution of ~20 to 30 nm. Despite the substantial progress in imaging chromatin organization in cells and model systems, its routine application on assessing pathological tissue remains limited. It is, in part, hampered by the lack of simple labels that consistently generates high-quality STORM images on the highly processed clinical tissue. We developed a fast, simple, and robust small-molecule fluorescent probe—cyanine 5–conjugated Hoechst—for routine superresolution imaging of nanoscale nuclear architecture on clinical tissue. We demonstrated the biological and clinical significance of imaging superresolved chromatin structure in cancer development and its potential clinical utility for cancer risk stratification. American Association for the Advancement of Science 2022-03-04 /pmc/articles/PMC8896800/ /pubmed/35245126 http://dx.doi.org/10.1126/sciadv.abm8293 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Xu, Jianquan
Sun, Xuejiao
Kim, Kwangho
Brand, Rhonda M.
Hartman, Douglas
Ma, Hongqiang
Brand, Randall E.
Bai, Mingfeng
Liu, Yang
Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
title Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
title_full Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
title_fullStr Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
title_full_unstemmed Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
title_short Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
title_sort ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896800/
https://www.ncbi.nlm.nih.gov/pubmed/35245126
http://dx.doi.org/10.1126/sciadv.abm8293
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