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Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
Imaging chromatin organization at the molecular-scale resolution remains an important endeavor in basic and translational research. Stochastic optical reconstruction microscopy (STORM) is a powerful superresolution imaging technique to visualize nanoscale molecular organization down to the resolutio...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896800/ https://www.ncbi.nlm.nih.gov/pubmed/35245126 http://dx.doi.org/10.1126/sciadv.abm8293 |
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author | Xu, Jianquan Sun, Xuejiao Kim, Kwangho Brand, Rhonda M. Hartman, Douglas Ma, Hongqiang Brand, Randall E. Bai, Mingfeng Liu, Yang |
author_facet | Xu, Jianquan Sun, Xuejiao Kim, Kwangho Brand, Rhonda M. Hartman, Douglas Ma, Hongqiang Brand, Randall E. Bai, Mingfeng Liu, Yang |
author_sort | Xu, Jianquan |
collection | PubMed |
description | Imaging chromatin organization at the molecular-scale resolution remains an important endeavor in basic and translational research. Stochastic optical reconstruction microscopy (STORM) is a powerful superresolution imaging technique to visualize nanoscale molecular organization down to the resolution of ~20 to 30 nm. Despite the substantial progress in imaging chromatin organization in cells and model systems, its routine application on assessing pathological tissue remains limited. It is, in part, hampered by the lack of simple labels that consistently generates high-quality STORM images on the highly processed clinical tissue. We developed a fast, simple, and robust small-molecule fluorescent probe—cyanine 5–conjugated Hoechst—for routine superresolution imaging of nanoscale nuclear architecture on clinical tissue. We demonstrated the biological and clinical significance of imaging superresolved chromatin structure in cancer development and its potential clinical utility for cancer risk stratification. |
format | Online Article Text |
id | pubmed-8896800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-88968002022-03-14 Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe Xu, Jianquan Sun, Xuejiao Kim, Kwangho Brand, Rhonda M. Hartman, Douglas Ma, Hongqiang Brand, Randall E. Bai, Mingfeng Liu, Yang Sci Adv Biomedicine and Life Sciences Imaging chromatin organization at the molecular-scale resolution remains an important endeavor in basic and translational research. Stochastic optical reconstruction microscopy (STORM) is a powerful superresolution imaging technique to visualize nanoscale molecular organization down to the resolution of ~20 to 30 nm. Despite the substantial progress in imaging chromatin organization in cells and model systems, its routine application on assessing pathological tissue remains limited. It is, in part, hampered by the lack of simple labels that consistently generates high-quality STORM images on the highly processed clinical tissue. We developed a fast, simple, and robust small-molecule fluorescent probe—cyanine 5–conjugated Hoechst—for routine superresolution imaging of nanoscale nuclear architecture on clinical tissue. We demonstrated the biological and clinical significance of imaging superresolved chromatin structure in cancer development and its potential clinical utility for cancer risk stratification. American Association for the Advancement of Science 2022-03-04 /pmc/articles/PMC8896800/ /pubmed/35245126 http://dx.doi.org/10.1126/sciadv.abm8293 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Xu, Jianquan Sun, Xuejiao Kim, Kwangho Brand, Rhonda M. Hartman, Douglas Ma, Hongqiang Brand, Randall E. Bai, Mingfeng Liu, Yang Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe |
title | Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe |
title_full | Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe |
title_fullStr | Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe |
title_full_unstemmed | Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe |
title_short | Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe |
title_sort | ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896800/ https://www.ncbi.nlm.nih.gov/pubmed/35245126 http://dx.doi.org/10.1126/sciadv.abm8293 |
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