Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing

SARS-CoV-2 has kept the world in suspense for almost 2 years now. The virus spread rapidly worldwide and several variants of concern have emerged: Alpha, Beta, Gamma, Delta and recently Omicron. A rapid method to detect key mutations is needed because these variants may jeopardize the effectiveness...

Descripción completa

Detalles Bibliográficos
Autores principales: Bloemen, Mandy, Rector, Annabel, Swinnen, Jill, Ranst, Marc Van, Maes, Piet, Vanmechelen, Bert, Wollants, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896866/
https://www.ncbi.nlm.nih.gov/pubmed/35257682
http://dx.doi.org/10.1016/j.jviromet.2022.114512
_version_ 1784663261431463936
author Bloemen, Mandy
Rector, Annabel
Swinnen, Jill
Ranst, Marc Van
Maes, Piet
Vanmechelen, Bert
Wollants, Elke
author_facet Bloemen, Mandy
Rector, Annabel
Swinnen, Jill
Ranst, Marc Van
Maes, Piet
Vanmechelen, Bert
Wollants, Elke
author_sort Bloemen, Mandy
collection PubMed
description SARS-CoV-2 has kept the world in suspense for almost 2 years now. The virus spread rapidly worldwide and several variants of concern have emerged: Alpha, Beta, Gamma, Delta and recently Omicron. A rapid method to detect key mutations is needed because these variants may jeopardize the effectiveness of immune protection following vaccination or past infection. This article describes an easy, cheap and fast method for the detection of mutations in the spike protein that are indicative for specific variants. This method can easily distinguish Omicron from other variants.
format Online
Article
Text
id pubmed-8896866
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-88968662022-03-07 Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing Bloemen, Mandy Rector, Annabel Swinnen, Jill Ranst, Marc Van Maes, Piet Vanmechelen, Bert Wollants, Elke J Virol Methods Article SARS-CoV-2 has kept the world in suspense for almost 2 years now. The virus spread rapidly worldwide and several variants of concern have emerged: Alpha, Beta, Gamma, Delta and recently Omicron. A rapid method to detect key mutations is needed because these variants may jeopardize the effectiveness of immune protection following vaccination or past infection. This article describes an easy, cheap and fast method for the detection of mutations in the spike protein that are indicative for specific variants. This method can easily distinguish Omicron from other variants. Elsevier B.V. 2022-06 2022-03-04 /pmc/articles/PMC8896866/ /pubmed/35257682 http://dx.doi.org/10.1016/j.jviromet.2022.114512 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Bloemen, Mandy
Rector, Annabel
Swinnen, Jill
Ranst, Marc Van
Maes, Piet
Vanmechelen, Bert
Wollants, Elke
Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
title Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
title_full Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
title_fullStr Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
title_full_unstemmed Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
title_short Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
title_sort fast detection of sars-cov-2 variants including omicron using one-step rt-pcr and sanger sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896866/
https://www.ncbi.nlm.nih.gov/pubmed/35257682
http://dx.doi.org/10.1016/j.jviromet.2022.114512
work_keys_str_mv AT bloemenmandy fastdetectionofsarscov2variantsincludingomicronusingonesteprtpcrandsangersequencing
AT rectorannabel fastdetectionofsarscov2variantsincludingomicronusingonesteprtpcrandsangersequencing
AT swinnenjill fastdetectionofsarscov2variantsincludingomicronusingonesteprtpcrandsangersequencing
AT ranstmarcvan fastdetectionofsarscov2variantsincludingomicronusingonesteprtpcrandsangersequencing
AT maespiet fastdetectionofsarscov2variantsincludingomicronusingonesteprtpcrandsangersequencing
AT vanmechelenbert fastdetectionofsarscov2variantsincludingomicronusingonesteprtpcrandsangersequencing
AT wollantselke fastdetectionofsarscov2variantsincludingomicronusingonesteprtpcrandsangersequencing

Ejemplares similares