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Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic

Designing highly active nanozymes for various enzymatic reactions remains a challenge in practical applications and fundamental research. In this work, by studying the catalytic functions of natural NADH oxidase (NOX), we devised and synthesized a porous carbon-supported cobalt catalyst (Co/C) to mi...

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Autores principales: Chen, Jinxing, Zheng, Xiliang, Zhang, Jiaxin, Ma, Qian, Zhao, Zhiwei, Huang, Liang, Wu, Weiwei, Wang, Ying, Wang, Jin, Dong, Shaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897313/
https://www.ncbi.nlm.nih.gov/pubmed/35261777
http://dx.doi.org/10.1093/nsr/nwab186
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author Chen, Jinxing
Zheng, Xiliang
Zhang, Jiaxin
Ma, Qian
Zhao, Zhiwei
Huang, Liang
Wu, Weiwei
Wang, Ying
Wang, Jin
Dong, Shaojun
author_facet Chen, Jinxing
Zheng, Xiliang
Zhang, Jiaxin
Ma, Qian
Zhao, Zhiwei
Huang, Liang
Wu, Weiwei
Wang, Ying
Wang, Jin
Dong, Shaojun
author_sort Chen, Jinxing
collection PubMed
description Designing highly active nanozymes for various enzymatic reactions remains a challenge in practical applications and fundamental research. In this work, by studying the catalytic functions of natural NADH oxidase (NOX), we devised and synthesized a porous carbon-supported cobalt catalyst (Co/C) to mimic NOX. The Co/C can catalyze dehydrogenation of NADH and transfers electrons to O(2) to produce H(2)O(2). Density functional theory calculations reveal that the Co/C can catalyze O(2) reduction to H(2)O(2) or H(2)O considerably. The Co/C can also mediate electron transfer from NADH to heme protein cytochrome c, thereby exhibiting cytochrome c reductase-like activity. The Co/C nanoparticles can deplete NADH in cancer cells, induce increase of the reactive oxygen species, lead to impairment of oxidative phosphorylation and decrease in mitochondrial membrane potential, and cause ATP production to be damaged. This ‘domino effect’ facilitates the cell to approach apoptosis.
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spelling pubmed-88973132022-03-07 Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic Chen, Jinxing Zheng, Xiliang Zhang, Jiaxin Ma, Qian Zhao, Zhiwei Huang, Liang Wu, Weiwei Wang, Ying Wang, Jin Dong, Shaojun Natl Sci Rev RESEARCH ARTICLE Designing highly active nanozymes for various enzymatic reactions remains a challenge in practical applications and fundamental research. In this work, by studying the catalytic functions of natural NADH oxidase (NOX), we devised and synthesized a porous carbon-supported cobalt catalyst (Co/C) to mimic NOX. The Co/C can catalyze dehydrogenation of NADH and transfers electrons to O(2) to produce H(2)O(2). Density functional theory calculations reveal that the Co/C can catalyze O(2) reduction to H(2)O(2) or H(2)O considerably. The Co/C can also mediate electron transfer from NADH to heme protein cytochrome c, thereby exhibiting cytochrome c reductase-like activity. The Co/C nanoparticles can deplete NADH in cancer cells, induce increase of the reactive oxygen species, lead to impairment of oxidative phosphorylation and decrease in mitochondrial membrane potential, and cause ATP production to be damaged. This ‘domino effect’ facilitates the cell to approach apoptosis. Oxford University Press 2021-10-11 /pmc/articles/PMC8897313/ /pubmed/35261777 http://dx.doi.org/10.1093/nsr/nwab186 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RESEARCH ARTICLE
Chen, Jinxing
Zheng, Xiliang
Zhang, Jiaxin
Ma, Qian
Zhao, Zhiwei
Huang, Liang
Wu, Weiwei
Wang, Ying
Wang, Jin
Dong, Shaojun
Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic
title Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic
title_full Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic
title_fullStr Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic
title_full_unstemmed Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic
title_short Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic
title_sort bubble-templated synthesis of nanocatalyst co/c as nadh oxidase mimic
topic RESEARCH ARTICLE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897313/
https://www.ncbi.nlm.nih.gov/pubmed/35261777
http://dx.doi.org/10.1093/nsr/nwab186
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