GBS-MeDIP: A protocol for parallel identification of genetic and epigenetic variation in the same reduced fraction of genomes across individuals

The GBS-MeDIP protocol combines two previously described techniques, Genotype-by-Sequencing (GBS) and Methylated-DNA-Immunoprecipitation (MeDIP). Our method allows for parallel and cost-efficient interrogation of genetic and methylomic variants in the DNA of many reduced genomes, taking advantage of...

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Detalles Bibliográficos
Autores principales: Rezaei, Shiva, Uffenorde, Julia, Gimm, Oliver, Hosseinpour Feizi, Mohammad Ali, Miemczyk, Stefan, Coutinho, Luiz Lehmann, Jensen, Per, Guerrero-Bosagna, Carlos, Pértille, Fábio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897576/
https://www.ncbi.nlm.nih.gov/pubmed/35257114
http://dx.doi.org/10.1016/j.xpro.2022.101202
Descripción
Sumario:The GBS-MeDIP protocol combines two previously described techniques, Genotype-by-Sequencing (GBS) and Methylated-DNA-Immunoprecipitation (MeDIP). Our method allows for parallel and cost-efficient interrogation of genetic and methylomic variants in the DNA of many reduced genomes, taking advantage of the barcoding of DNA samples performed in the GBS and the subsequent creation of DNA pools, then used as an input for the MeDIP. The GBS-MeDIP is particularly suitable to identify genetic and methylomic biomarkers when resources for whole genome interrogation are lacking.

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