Protocol for using negative pressure isolator systems to study BSL-2 organisms in gnotobiotic murine models

Here, we present a protocol for the use of negative pressure isolator systems to maintain defined association and contain BSL-2 pathogens in germ-free and gnotobiotic mouse studies. We describe setup and operation of negative pressure isolators with integrated microbiologic procedures, using the BSL...

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Detalles Bibliográficos
Autores principales: Pavao, Aidan, Graham, Madeline, Trofimuk, Olivia, Delaney, Mary L., Yeliseyev, Vladimir, Bry, Lynn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897582/
https://www.ncbi.nlm.nih.gov/pubmed/35257115
http://dx.doi.org/10.1016/j.xpro.2022.101211

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