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High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy

Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating...

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Detalles Bibliográficos
Autor principal: Grillet, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897606/
https://www.ncbi.nlm.nih.gov/pubmed/35257116
http://dx.doi.org/10.1016/j.xpro.2022.101213
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author Grillet, Nicolas
author_facet Grillet, Nicolas
author_sort Grillet, Nicolas
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description Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse’s inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).
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spelling pubmed-88976062022-03-06 High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy Grillet, Nicolas STAR Protoc Protocol Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse’s inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021). Elsevier 2022-03-03 /pmc/articles/PMC8897606/ /pubmed/35257116 http://dx.doi.org/10.1016/j.xpro.2022.101213 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Grillet, Nicolas
High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
title High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
title_full High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
title_fullStr High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
title_full_unstemmed High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
title_short High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
title_sort high-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897606/
https://www.ncbi.nlm.nih.gov/pubmed/35257116
http://dx.doi.org/10.1016/j.xpro.2022.101213
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