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High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy
Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897606/ https://www.ncbi.nlm.nih.gov/pubmed/35257116 http://dx.doi.org/10.1016/j.xpro.2022.101213 |
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author | Grillet, Nicolas |
author_facet | Grillet, Nicolas |
author_sort | Grillet, Nicolas |
collection | PubMed |
description | Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse’s inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021). |
format | Online Article Text |
id | pubmed-8897606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-88976062022-03-06 High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy Grillet, Nicolas STAR Protoc Protocol Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse’s inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021). Elsevier 2022-03-03 /pmc/articles/PMC8897606/ /pubmed/35257116 http://dx.doi.org/10.1016/j.xpro.2022.101213 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Grillet, Nicolas High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy |
title | High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy |
title_full | High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy |
title_fullStr | High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy |
title_full_unstemmed | High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy |
title_short | High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy |
title_sort | high-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897606/ https://www.ncbi.nlm.nih.gov/pubmed/35257116 http://dx.doi.org/10.1016/j.xpro.2022.101213 |
work_keys_str_mv | AT grilletnicolas highresolutionimagingofthemousehaircellhairbundlebyscanningelectronmicroscopy |