Effect of prooxidants and chelator Desferal on the oxidative status and sperm motility of Muscovy semen

This study aimed to establish the sensitivity of Muscovy duck semen to oxidative stress (OS) and the effect of Desferal, applied as an antioxidant. The effect of three prooxidant systems in presence and absence of Desferal were tested on the motility and kinetic parameters (determined using CASA system), as well as the level of lipid peroxidation (LPO) and glutathione (tGSH) of Muscovy semen. The semen was diluted (1:3 v/v) with four extenders (saline solution, IMV Canadyl, HIA-1, and AU) and stored at 4 °C for 6 h. The cooled semen was divided into aliquots (50 × 10(6) sperm cells/mL), which were incubated at 37 °C for 30 min with one of the following prooxidative agents: ferrous sulfate (FeSO(4,) 0.1 mM), hydrogen peroxide (H(2)O(2), 1 mM), and Fenton system (FeSO(4)(Fe(2+)), 0.1 mM + H(2)O(2), 1 mM), in the presence or absence of Desferal (0.1 mM). The addition of FeSO(4) + H(2)O(2) or FeSO(4) regardless of the used extender, as well as the addition of H(2)O(2) to the diluted semen with saline solution significantly increased the levels of LPO (P < 0.05). With the lowest prooxidant effect was H(2)O(2). The application of Desferal reduced significantly (P < 0.05) the LPO levels induced by FeSO(4) + H(2)O(2) or FeSO(4) and in a weaker degree by H(2)O(2). Among all prooxidants, FeSO(4) + H(2)O(2) decreased in the greatest extent the tGSH concentration in semen diluted with each used extenders in comparison to the relevant control. The addition of Desferal in semen diluted with HIA-1 extender and incubated with FeSO(4), and H(2)O(2), showed the best restoration of tGSH level, close to that of respectively controls. The studied prooxidants significantly reduced total, progressive, and kinetic sperm motility (P < 0.05). Although the inclusion of Desferal reduced the sperm OS, it did not improve the impaired by OS sperm motility.