Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol

The paper reports the spectrofluorimetric and calorimetric study of binding of two hydrophobic biologically active molecules with antioxidant ability, flavonoids quercetin, and curcumin, to human serum albumin (HSA) in water, aqueous DMSO (0.05 and 0.1 mol. fraction of DMSO), and aqueous ethanol (0....

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Autores principales: Usacheva, Tatyana, Gamov, George, Bychkova, Anna, Anufrikov, Yuriy, Shasherina, Anna, Alister, Diana, Kuranova, Natalya, Sharnin, Valentin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897738/
https://www.ncbi.nlm.nih.gov/pubmed/35283663
http://dx.doi.org/10.1007/s10973-022-11216-8
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author Usacheva, Tatyana
Gamov, George
Bychkova, Anna
Anufrikov, Yuriy
Shasherina, Anna
Alister, Diana
Kuranova, Natalya
Sharnin, Valentin
author_facet Usacheva, Tatyana
Gamov, George
Bychkova, Anna
Anufrikov, Yuriy
Shasherina, Anna
Alister, Diana
Kuranova, Natalya
Sharnin, Valentin
author_sort Usacheva, Tatyana
collection PubMed
description The paper reports the spectrofluorimetric and calorimetric study of binding of two hydrophobic biologically active molecules with antioxidant ability, flavonoids quercetin, and curcumin, to human serum albumin (HSA) in water, aqueous DMSO (0.05 and 0.1 mol. fraction of DMSO), and aqueous ethanol (0.05 mol. fraction of EtOH). Both flavonoids induce the quenching of HSA fluorescence. The stability constants of associates, as well as the changes in enthalpy of the reaction between quercetin and protein, were evaluated. The influence of solvent composition and additions of hydroxypropyl-β-cyclodextrin as a solubilizer of hydrophobic molecules, on the association processes is discussed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10973-022-11216-8.
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spelling pubmed-88977382022-03-07 Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol Usacheva, Tatyana Gamov, George Bychkova, Anna Anufrikov, Yuriy Shasherina, Anna Alister, Diana Kuranova, Natalya Sharnin, Valentin J Therm Anal Calorim Article The paper reports the spectrofluorimetric and calorimetric study of binding of two hydrophobic biologically active molecules with antioxidant ability, flavonoids quercetin, and curcumin, to human serum albumin (HSA) in water, aqueous DMSO (0.05 and 0.1 mol. fraction of DMSO), and aqueous ethanol (0.05 mol. fraction of EtOH). Both flavonoids induce the quenching of HSA fluorescence. The stability constants of associates, as well as the changes in enthalpy of the reaction between quercetin and protein, were evaluated. The influence of solvent composition and additions of hydroxypropyl-β-cyclodextrin as a solubilizer of hydrophobic molecules, on the association processes is discussed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10973-022-11216-8. Springer International Publishing 2022-03-05 2022 /pmc/articles/PMC8897738/ /pubmed/35283663 http://dx.doi.org/10.1007/s10973-022-11216-8 Text en © Akadémiai Kiadó, Budapest, Hungary 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Usacheva, Tatyana
Gamov, George
Bychkova, Anna
Anufrikov, Yuriy
Shasherina, Anna
Alister, Diana
Kuranova, Natalya
Sharnin, Valentin
Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol
title Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol
title_full Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol
title_fullStr Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol
title_full_unstemmed Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol
title_short Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol
title_sort binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897738/
https://www.ncbi.nlm.nih.gov/pubmed/35283663
http://dx.doi.org/10.1007/s10973-022-11216-8
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