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Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin

PURPOSE: Gastric injury is a major issue for long-term administration of aspirin. In this work, we tried to explore the possibility of using BLG to alleviate aspirin-induced gastric injury, because of excellent abilities of BLG in loading drug molecules. METHODS: Various spectroscopic techniques and...

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Autores principales: Chen, Jin, Gong, Min, Huang, Zhuo, Wang, Fang, Wang, Yajing, Hu, Zuquan, Zeng, Zhu, Wang, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898184/
https://www.ncbi.nlm.nih.gov/pubmed/35256843
http://dx.doi.org/10.2147/DDDT.S351100
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author Chen, Jin
Gong, Min
Huang, Zhuo
Wang, Fang
Wang, Yajing
Hu, Zuquan
Zeng, Zhu
Wang, Yun
author_facet Chen, Jin
Gong, Min
Huang, Zhuo
Wang, Fang
Wang, Yajing
Hu, Zuquan
Zeng, Zhu
Wang, Yun
author_sort Chen, Jin
collection PubMed
description PURPOSE: Gastric injury is a major issue for long-term administration of aspirin. In this work, we tried to explore the possibility of using BLG to alleviate aspirin-induced gastric injury, because of excellent abilities of BLG in loading drug molecules. METHODS: Various spectroscopic techniques and molecular docking methods were applied to investigate the interaction mechanism between BLG and aspirin. Animal experiments were performed to figure out the effects of taking aspirin-BLG on the stomach. RESULTS: Our results demonstrate that aspirin could bind with BLG to form stable aspirin-BLG complex (the binding constant K(b)= 2.051 × 10(3) M(−1)). The formation process is endothermic (∆H>0) and the main acting force is hydrophobic force. Our data also show that the aspirin-BLG complex is formed with a higher affinity in simulated gastric fluid and could remain stable for several hours, which might arise from its special binding mode under acidic condition and the resistance of BLG to gastric digestion. Furthermore, animal models (rats with aspirin-induced gastric damage) were built. The results of animal experiments reveal that the oral administration of aspirin-BLG could cause less damage to gastric tissue, and it also hardly triggers obvious inflammatory responses. CONCLUSION: This study would contribute to an in-depth understanding of the interaction mechanism between BLG and aspirin. It is reasonable to believe that using BLG to bind with aspirin would be a potential way to alleviate the aspirin-induced gastric injury.
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spelling pubmed-88981842022-03-06 Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin Chen, Jin Gong, Min Huang, Zhuo Wang, Fang Wang, Yajing Hu, Zuquan Zeng, Zhu Wang, Yun Drug Des Devel Ther Original Research PURPOSE: Gastric injury is a major issue for long-term administration of aspirin. In this work, we tried to explore the possibility of using BLG to alleviate aspirin-induced gastric injury, because of excellent abilities of BLG in loading drug molecules. METHODS: Various spectroscopic techniques and molecular docking methods were applied to investigate the interaction mechanism between BLG and aspirin. Animal experiments were performed to figure out the effects of taking aspirin-BLG on the stomach. RESULTS: Our results demonstrate that aspirin could bind with BLG to form stable aspirin-BLG complex (the binding constant K(b)= 2.051 × 10(3) M(−1)). The formation process is endothermic (∆H>0) and the main acting force is hydrophobic force. Our data also show that the aspirin-BLG complex is formed with a higher affinity in simulated gastric fluid and could remain stable for several hours, which might arise from its special binding mode under acidic condition and the resistance of BLG to gastric digestion. Furthermore, animal models (rats with aspirin-induced gastric damage) were built. The results of animal experiments reveal that the oral administration of aspirin-BLG could cause less damage to gastric tissue, and it also hardly triggers obvious inflammatory responses. CONCLUSION: This study would contribute to an in-depth understanding of the interaction mechanism between BLG and aspirin. It is reasonable to believe that using BLG to bind with aspirin would be a potential way to alleviate the aspirin-induced gastric injury. Dove 2022-03-01 /pmc/articles/PMC8898184/ /pubmed/35256843 http://dx.doi.org/10.2147/DDDT.S351100 Text en © 2022 Chen et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Chen, Jin
Gong, Min
Huang, Zhuo
Wang, Fang
Wang, Yajing
Hu, Zuquan
Zeng, Zhu
Wang, Yun
Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin
title Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin
title_full Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin
title_fullStr Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin
title_full_unstemmed Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin
title_short Alleviating Aspirin-Induced Gastric Injury by Binding Aspirin to β-Lactoglobulin
title_sort alleviating aspirin-induced gastric injury by binding aspirin to β-lactoglobulin
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898184/
https://www.ncbi.nlm.nih.gov/pubmed/35256843
http://dx.doi.org/10.2147/DDDT.S351100
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