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RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression

Chemoresistance is closely related to the therapeutic effect and prognosis in breast cancer patients. Increasing evidences demonstrated that RNA binding proteins (RBPs) have notable roles in regulating cancer cell proliferation, metastasis and chemotherapeutic sensitivity. RNA binding motif single s...

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Autores principales: Xu, Feng, Xia, Tian, Xu, Qi-Tong, Zhang, Xu, Huang, Yu-Zhou, Sun, Xi, Shi, Liang, Zhou, Xu-Jie, Wei, Ji-Fu, Ding, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898364/
https://www.ncbi.nlm.nih.gov/pubmed/35280673
http://dx.doi.org/10.7150/ijbs.66480
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author Xu, Feng
Xia, Tian
Xu, Qi-Tong
Zhang, Xu
Huang, Yu-Zhou
Sun, Xi
Shi, Liang
Zhou, Xu-Jie
Wei, Ji-Fu
Ding, Qiang
author_facet Xu, Feng
Xia, Tian
Xu, Qi-Tong
Zhang, Xu
Huang, Yu-Zhou
Sun, Xi
Shi, Liang
Zhou, Xu-Jie
Wei, Ji-Fu
Ding, Qiang
author_sort Xu, Feng
collection PubMed
description Chemoresistance is closely related to the therapeutic effect and prognosis in breast cancer patients. Increasing evidences demonstrated that RNA binding proteins (RBPs) have notable roles in regulating cancer cell proliferation, metastasis and chemotherapeutic sensitivity. RNA binding motif single stranded interacting protein 2 (RBMS2), an RBP, has been considered to be a tumor suppressor in several cancers. However, its role of doxorubicin sensitivity in breast cancer patients has not yet been fully revealed. Here, we performed doxorubicin cytotoxicity assay, flow cytometry and mouse xenograft model to examine the influence of RBMS2 on doxorubicin sensitization in vitro and in vivo. RIP assay and dual-luciferase reporter assay were performed to explore the relationship between RBMS2 and BMF. Our data demonstrated that upregulation of RBMS2 in breast cancer cells could enhance sensitivity to doxorubicin and promote apoptosis in the presence of doxorubicin, while inhibition of RBMS2 showed an opposite trend. Moreover, this chemosensitizing effect of RBMS2 could be reversed by the inhibition of Bcl-2 modifying factor (BMF). RBMS2 positively regulated BMF expression and increased BMF-induced expression of (cleaved) caspase 3, (cleaved) caspase 9 and poly (ADP-Ribose) polymerase (PARP). These results uncovered a novel mechanism for RBMS2 in the sensibilization of doxorubicin, suggesting that RBMS2 may act as a potential therapeutic target for drug-resistant breast cancer.
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spelling pubmed-88983642022-03-10 RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression Xu, Feng Xia, Tian Xu, Qi-Tong Zhang, Xu Huang, Yu-Zhou Sun, Xi Shi, Liang Zhou, Xu-Jie Wei, Ji-Fu Ding, Qiang Int J Biol Sci Research Paper Chemoresistance is closely related to the therapeutic effect and prognosis in breast cancer patients. Increasing evidences demonstrated that RNA binding proteins (RBPs) have notable roles in regulating cancer cell proliferation, metastasis and chemotherapeutic sensitivity. RNA binding motif single stranded interacting protein 2 (RBMS2), an RBP, has been considered to be a tumor suppressor in several cancers. However, its role of doxorubicin sensitivity in breast cancer patients has not yet been fully revealed. Here, we performed doxorubicin cytotoxicity assay, flow cytometry and mouse xenograft model to examine the influence of RBMS2 on doxorubicin sensitization in vitro and in vivo. RIP assay and dual-luciferase reporter assay were performed to explore the relationship between RBMS2 and BMF. Our data demonstrated that upregulation of RBMS2 in breast cancer cells could enhance sensitivity to doxorubicin and promote apoptosis in the presence of doxorubicin, while inhibition of RBMS2 showed an opposite trend. Moreover, this chemosensitizing effect of RBMS2 could be reversed by the inhibition of Bcl-2 modifying factor (BMF). RBMS2 positively regulated BMF expression and increased BMF-induced expression of (cleaved) caspase 3, (cleaved) caspase 9 and poly (ADP-Ribose) polymerase (PARP). These results uncovered a novel mechanism for RBMS2 in the sensibilization of doxorubicin, suggesting that RBMS2 may act as a potential therapeutic target for drug-resistant breast cancer. Ivyspring International Publisher 2022-02-07 /pmc/articles/PMC8898364/ /pubmed/35280673 http://dx.doi.org/10.7150/ijbs.66480 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Xu, Feng
Xia, Tian
Xu, Qi-Tong
Zhang, Xu
Huang, Yu-Zhou
Sun, Xi
Shi, Liang
Zhou, Xu-Jie
Wei, Ji-Fu
Ding, Qiang
RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression
title RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression
title_full RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression
title_fullStr RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression
title_full_unstemmed RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression
title_short RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression
title_sort rbms2 chemosensitizes breast cancer cells to doxorubicin by regulating bmf expression
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898364/
https://www.ncbi.nlm.nih.gov/pubmed/35280673
http://dx.doi.org/10.7150/ijbs.66480
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