Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins

Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of...

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Detalles Bibliográficos
Autores principales: Zhi, Zelun, Ma, Xuejing, Zhou, Chang, Mechler, Adam, Zhang, Shuyan, Liu, Pingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899027/
https://www.ncbi.nlm.nih.gov/pubmed/35265861
http://dx.doi.org/10.1016/j.xpro.2022.101214
Descripción
Sumario:Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of perilipin 2 on natural lipid droplets, construct artificial lipid droplets, and determine the binding affinity of perilipin 2 on artificial lipid droplets. This protocol can be adapted to determine the binding properties of various lipid droplet-associated proteins. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021).

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