Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins

Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of...

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Detalles Bibliográficos
Autores principales: Zhi, Zelun, Ma, Xuejing, Zhou, Chang, Mechler, Adam, Zhang, Shuyan, Liu, Pingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899027/
https://www.ncbi.nlm.nih.gov/pubmed/35265861
http://dx.doi.org/10.1016/j.xpro.2022.101214
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author Zhi, Zelun
Ma, Xuejing
Zhou, Chang
Mechler, Adam
Zhang, Shuyan
Liu, Pingsheng
author_facet Zhi, Zelun
Ma, Xuejing
Zhou, Chang
Mechler, Adam
Zhang, Shuyan
Liu, Pingsheng
author_sort Zhi, Zelun
collection PubMed
description Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of perilipin 2 on natural lipid droplets, construct artificial lipid droplets, and determine the binding affinity of perilipin 2 on artificial lipid droplets. This protocol can be adapted to determine the binding properties of various lipid droplet-associated proteins. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021).
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spelling pubmed-88990272022-03-08 Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins Zhi, Zelun Ma, Xuejing Zhou, Chang Mechler, Adam Zhang, Shuyan Liu, Pingsheng STAR Protoc Protocol Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of perilipin 2 on natural lipid droplets, construct artificial lipid droplets, and determine the binding affinity of perilipin 2 on artificial lipid droplets. This protocol can be adapted to determine the binding properties of various lipid droplet-associated proteins. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021). Elsevier 2022-03-03 /pmc/articles/PMC8899027/ /pubmed/35265861 http://dx.doi.org/10.1016/j.xpro.2022.101214 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhi, Zelun
Ma, Xuejing
Zhou, Chang
Mechler, Adam
Zhang, Shuyan
Liu, Pingsheng
Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins
title Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins
title_full Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins
title_fullStr Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins
title_full_unstemmed Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins
title_short Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins
title_sort protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899027/
https://www.ncbi.nlm.nih.gov/pubmed/35265861
http://dx.doi.org/10.1016/j.xpro.2022.101214
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