Use of prepubertal environment enrichment to prevent dopamine dysregulation in a neurodevelopmental rat model of schizophrenia risk

Here, we present a protocol for delivering environmental enrichment (EE) in discrete postnatal windows to prevent long-term dopamine neuron dysfunctions in a neurodevelopmental rat model of schizophrenia risk. We describe generation of the schizophrenia model through prenatal treatment of rats with methylazoxymethanol acetate (the MAM model) and the saline-treated controls. We then detail the 10-day or 20-day EE paradigms applied on male rats at different ages. This protocol also includes preparation of control groups in regular environment (RE) cages for comparison. For complete details on the use and execution of this protocol, please refer to Zhu and Grace (2021).