A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells

Alternative lengthening of telomeres (ALT) is a homologous recombination-based telomere maintenance mechanism. It is active in approximately 10–15% of cancers. We present a DNA-fiber protocol, combining YOYO-1 staining of genomic DNA, telomere fluorescence in situ hybridization (FISH), and EdU label...

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Detalles Bibliográficos
Autores principales: Lu, Robert, Allen, Joshua A.M., Galaviz, Pablo, Pickett, Hilda A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899046/
https://www.ncbi.nlm.nih.gov/pubmed/35265860
http://dx.doi.org/10.1016/j.xpro.2022.101212
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author Lu, Robert
Allen, Joshua A.M.
Galaviz, Pablo
Pickett, Hilda A.
author_facet Lu, Robert
Allen, Joshua A.M.
Galaviz, Pablo
Pickett, Hilda A.
author_sort Lu, Robert
collection PubMed
description Alternative lengthening of telomeres (ALT) is a homologous recombination-based telomere maintenance mechanism. It is active in approximately 10–15% of cancers. We present a DNA-fiber protocol, combining YOYO-1 staining of genomic DNA, telomere fluorescence in situ hybridization (FISH), and EdU labeling of nascent DNA, to measure telomere extension events in ALT cancer cells. The protocol can be used to delineate ALT-mediated telomere extension. For complete details on the use and execution of this protocol, please refer to Barroso-Gonzalez et al. (2021).
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spelling pubmed-88990462022-03-08 A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells Lu, Robert Allen, Joshua A.M. Galaviz, Pablo Pickett, Hilda A. STAR Protoc Protocol Alternative lengthening of telomeres (ALT) is a homologous recombination-based telomere maintenance mechanism. It is active in approximately 10–15% of cancers. We present a DNA-fiber protocol, combining YOYO-1 staining of genomic DNA, telomere fluorescence in situ hybridization (FISH), and EdU labeling of nascent DNA, to measure telomere extension events in ALT cancer cells. The protocol can be used to delineate ALT-mediated telomere extension. For complete details on the use and execution of this protocol, please refer to Barroso-Gonzalez et al. (2021). Elsevier 2022-03-04 /pmc/articles/PMC8899046/ /pubmed/35265860 http://dx.doi.org/10.1016/j.xpro.2022.101212 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lu, Robert
Allen, Joshua A.M.
Galaviz, Pablo
Pickett, Hilda A.
A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells
title A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells
title_full A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells
title_fullStr A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells
title_full_unstemmed A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells
title_short A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells
title_sort dna-fiber protocol for single molecule analysis of telomere (smat) length and extension events in cancer cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899046/
https://www.ncbi.nlm.nih.gov/pubmed/35265860
http://dx.doi.org/10.1016/j.xpro.2022.101212
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