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A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells

Alternative lengthening of telomeres (ALT) is a homologous recombination-based telomere maintenance mechanism. It is active in approximately 10–15% of cancers. We present a DNA-fiber protocol, combining YOYO-1 staining of genomic DNA, telomere fluorescence in situ hybridization (FISH), and EdU label...

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Detalles Bibliográficos
Autores principales:	Lu, Robert, Allen, Joshua A.M., Galaviz, Pablo, Pickett, Hilda A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899046/ https://www.ncbi.nlm.nih.gov/pubmed/35265860 http://dx.doi.org/10.1016/j.xpro.2022.101212

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