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Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer

Aptamers have excellent specificity and affinity in targeting cell surface receptors, showing great potential in targeted delivery of drugs, siRNA, mRNA, and various nanomaterials with therapeutic function. A better insight of the receptor-mediated internalization process of aptameric conjugates cou...

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Autores principales: Zhang, Nan, Wang, Junyan, Bing, Tao, Liu, Xiangjun, Shangguan, Dihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899136/
https://www.ncbi.nlm.nih.gov/pubmed/35282414
http://dx.doi.org/10.1016/j.omtn.2022.02.006
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author Zhang, Nan
Wang, Junyan
Bing, Tao
Liu, Xiangjun
Shangguan, Dihua
author_facet Zhang, Nan
Wang, Junyan
Bing, Tao
Liu, Xiangjun
Shangguan, Dihua
author_sort Zhang, Nan
collection PubMed
description Aptamers have excellent specificity and affinity in targeting cell surface receptors, showing great potential in targeted delivery of drugs, siRNA, mRNA, and various nanomaterials with therapeutic function. A better insight of the receptor-mediated internalization process of aptameric conjugates could facilitate the design of new targeted drugs. In this paper, human transferrin receptor-targeted DNA aptamer (termed HG1-9)-fluorophore conjugates were synthesized to visualize the internalization, intracellular transport, and nano-environmental pH of aptameric conjugates. Unlike transferrin that showed high recycling rate and short duration time in cells, the synthetic aptameric conjugates continuously accumulated within cells at a relatively slower rate, besides recycling back to cell surface. After long incubation (≥2 h), only very small amounts of HG1-9 conjugates (approximately 5%) entered late endosomes or lysosomes, and more than 90% of internalized HG1-9 was retained in cellular vesicles (pH 6.0–6.8), escaping from degradation. And among the internalized HG1-9 conjugates, approximately 20% was dissociated from transferrin receptor. The lower recycling ratios of HG1-9 conjugates and their dissociation from receptors promote the accurate and efficient release of their loaded drugs. These results suggest that aptamer HG1-9 could be provided as a versatile tool for specific and effective delivery of diverse therapeutic payloads.
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spelling pubmed-88991362022-03-11 Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer Zhang, Nan Wang, Junyan Bing, Tao Liu, Xiangjun Shangguan, Dihua Mol Ther Nucleic Acids Original Article Aptamers have excellent specificity and affinity in targeting cell surface receptors, showing great potential in targeted delivery of drugs, siRNA, mRNA, and various nanomaterials with therapeutic function. A better insight of the receptor-mediated internalization process of aptameric conjugates could facilitate the design of new targeted drugs. In this paper, human transferrin receptor-targeted DNA aptamer (termed HG1-9)-fluorophore conjugates were synthesized to visualize the internalization, intracellular transport, and nano-environmental pH of aptameric conjugates. Unlike transferrin that showed high recycling rate and short duration time in cells, the synthetic aptameric conjugates continuously accumulated within cells at a relatively slower rate, besides recycling back to cell surface. After long incubation (≥2 h), only very small amounts of HG1-9 conjugates (approximately 5%) entered late endosomes or lysosomes, and more than 90% of internalized HG1-9 was retained in cellular vesicles (pH 6.0–6.8), escaping from degradation. And among the internalized HG1-9 conjugates, approximately 20% was dissociated from transferrin receptor. The lower recycling ratios of HG1-9 conjugates and their dissociation from receptors promote the accurate and efficient release of their loaded drugs. These results suggest that aptamer HG1-9 could be provided as a versatile tool for specific and effective delivery of diverse therapeutic payloads. American Society of Gene & Cell Therapy 2022-02-12 /pmc/articles/PMC8899136/ /pubmed/35282414 http://dx.doi.org/10.1016/j.omtn.2022.02.006 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zhang, Nan
Wang, Junyan
Bing, Tao
Liu, Xiangjun
Shangguan, Dihua
Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer
title Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer
title_full Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer
title_fullStr Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer
title_full_unstemmed Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer
title_short Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer
title_sort transferrin receptor-mediated internalization and intracellular fate of conjugates of a dna aptamer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899136/
https://www.ncbi.nlm.nih.gov/pubmed/35282414
http://dx.doi.org/10.1016/j.omtn.2022.02.006
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