Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system

Mosaicism, including alleles comprising both wild-type and mutant, is a serious problem for gene modification by gene editing using electroporation. One-step generation of F0 pigs with completely desired gene modifications saves cost and time, but the major obstacles have been mosaic mutations. We h...

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Detalles Bibliográficos
Autores principales: Namula, Zhao, Wittayarat, Manita, Do, Lanh Thi Kim, Van Nguyen, Thanh, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Tanihara, Fuminori, Otoi, Takeshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899406/
https://www.ncbi.nlm.nih.gov/pubmed/35265771
http://dx.doi.org/10.1016/j.vas.2022.100241

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899406/
https://www.ncbi.nlm.nih.gov/pubmed/35265771
http://dx.doi.org/10.1016/j.vas.2022.100241

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