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Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis
Psoriasis is a complex chronic inflammatory skin disease with unclear molecular mechanisms. We found that the Src homology‐2 domain‐containing protein tyrosine phosphatase‐2 (SHP2) was highly expressed in both psoriatic patients and imiquimod (IMQ)‐induced psoriasis‐like mice. Also, the SHP2 alloste...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899919/ https://www.ncbi.nlm.nih.gov/pubmed/34936223 http://dx.doi.org/10.15252/emmm.202114455 |
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author | Zhu, Yuyu Wu, Zhigui Yan, Wei Shao, Fenli Ke, Bowen Jiang, Xian Gao, Jian Guo, Wenjie Lai, Yuping Ma, Hongyue Chen, Dijun Xu, Qiang Sun, Yang |
author_facet | Zhu, Yuyu Wu, Zhigui Yan, Wei Shao, Fenli Ke, Bowen Jiang, Xian Gao, Jian Guo, Wenjie Lai, Yuping Ma, Hongyue Chen, Dijun Xu, Qiang Sun, Yang |
author_sort | Zhu, Yuyu |
collection | PubMed |
description | Psoriasis is a complex chronic inflammatory skin disease with unclear molecular mechanisms. We found that the Src homology‐2 domain‐containing protein tyrosine phosphatase‐2 (SHP2) was highly expressed in both psoriatic patients and imiquimod (IMQ)‐induced psoriasis‐like mice. Also, the SHP2 allosteric inhibitor SHP099 reduced pro‐inflammatory cytokine expression in PBMCs taken from psoriatic patients. Consistently, SHP099 significantly ameliorated IMQ‐triggered skin inflammation in mice. Single‐cell RNA sequencing of murine skin demonstrated that SHP2 inhibition impaired skin inflammation in myeloid cells, especially macrophages. Furthermore, IMQ‐induced psoriasis‐like skin inflammation was significantly alleviated in myeloid cells (monocytes, mature macrophages, and granulocytes)—but not dendritic cells conditional SHP2 knockout mice. Mechanistically, SHP2 promoted the trafficking of toll‐like receptor 7 (TLR7) from the Golgi to the endosome in macrophages by dephosphorylating TLR7 at Tyr1024, boosting the ubiquitination of TLR7 and NF‐κB‐mediated skin inflammation. Importantly, Tlr7 point‐mutant knock‐in mice showed an attenuated psoriasis‐like phenotype compared to wild‐type littermates following IMQ treatment. Collectively, our findings identify SHP2 as a novel regulator of psoriasis and suggest that SHP2 inhibition may be a promising therapeutic approach for psoriatic patients. |
format | Online Article Text |
id | pubmed-8899919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88999192022-03-11 Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis Zhu, Yuyu Wu, Zhigui Yan, Wei Shao, Fenli Ke, Bowen Jiang, Xian Gao, Jian Guo, Wenjie Lai, Yuping Ma, Hongyue Chen, Dijun Xu, Qiang Sun, Yang EMBO Mol Med Articles Psoriasis is a complex chronic inflammatory skin disease with unclear molecular mechanisms. We found that the Src homology‐2 domain‐containing protein tyrosine phosphatase‐2 (SHP2) was highly expressed in both psoriatic patients and imiquimod (IMQ)‐induced psoriasis‐like mice. Also, the SHP2 allosteric inhibitor SHP099 reduced pro‐inflammatory cytokine expression in PBMCs taken from psoriatic patients. Consistently, SHP099 significantly ameliorated IMQ‐triggered skin inflammation in mice. Single‐cell RNA sequencing of murine skin demonstrated that SHP2 inhibition impaired skin inflammation in myeloid cells, especially macrophages. Furthermore, IMQ‐induced psoriasis‐like skin inflammation was significantly alleviated in myeloid cells (monocytes, mature macrophages, and granulocytes)—but not dendritic cells conditional SHP2 knockout mice. Mechanistically, SHP2 promoted the trafficking of toll‐like receptor 7 (TLR7) from the Golgi to the endosome in macrophages by dephosphorylating TLR7 at Tyr1024, boosting the ubiquitination of TLR7 and NF‐κB‐mediated skin inflammation. Importantly, Tlr7 point‐mutant knock‐in mice showed an attenuated psoriasis‐like phenotype compared to wild‐type littermates following IMQ treatment. Collectively, our findings identify SHP2 as a novel regulator of psoriasis and suggest that SHP2 inhibition may be a promising therapeutic approach for psoriatic patients. John Wiley and Sons Inc. 2021-12-22 2022-03-07 /pmc/articles/PMC8899919/ /pubmed/34936223 http://dx.doi.org/10.15252/emmm.202114455 Text en © 2021 The Authors. Published under the terms of the CC BY 4.0 license https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Zhu, Yuyu Wu, Zhigui Yan, Wei Shao, Fenli Ke, Bowen Jiang, Xian Gao, Jian Guo, Wenjie Lai, Yuping Ma, Hongyue Chen, Dijun Xu, Qiang Sun, Yang Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis |
title | Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis |
title_full | Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis |
title_fullStr | Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis |
title_full_unstemmed | Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis |
title_short | Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis |
title_sort | allosteric inhibition of shp2 uncovers aberrant tlr7 trafficking in aggravating psoriasis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899919/ https://www.ncbi.nlm.nih.gov/pubmed/34936223 http://dx.doi.org/10.15252/emmm.202114455 |
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