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Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM

OBJECTIVE: Micro RNAs (miRNAs) are gaining acceptance as novel biomarkers for the autoimmune disorders. However, miRNA profiles have not been investigated in individuals at risk of or diagnosed with type 1 diabetes mellitus (T1DM). To study the expression pattern of miRNAs in plasma obtained from pa...

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Autores principales: Barseem, Naglaa Fathy, Mahasab, Marwa Mohamed, Zaed, Ibrahem Fathy, Said, Aya Eldesoky A., El Gayed, Eman Masoud Abd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Galenos Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900082/
https://www.ncbi.nlm.nih.gov/pubmed/34927407
http://dx.doi.org/10.4274/jcrpe.galenos.2021.2021.0080
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author Barseem, Naglaa Fathy
Mahasab, Marwa Mohamed
Zaed, Ibrahem Fathy
Said, Aya Eldesoky A.
El Gayed, Eman Masoud Abd
author_facet Barseem, Naglaa Fathy
Mahasab, Marwa Mohamed
Zaed, Ibrahem Fathy
Said, Aya Eldesoky A.
El Gayed, Eman Masoud Abd
author_sort Barseem, Naglaa Fathy
collection PubMed
description OBJECTIVE: Micro RNAs (miRNAs) are gaining acceptance as novel biomarkers for the autoimmune disorders. However, miRNA profiles have not been investigated in individuals at risk of or diagnosed with type 1 diabetes mellitus (T1DM). To study the expression pattern of miRNAs in plasma obtained from patients with T1DM and compare with matched healthy controls. METHODS: Equal numbers of patients with T1DM (90) and healthy-matched control children (90) were assessed for the expression profile of plasma miRNAs including miRNA-101-5p, miRNA-146-5p, miRNA-21-5p, miRNA-375, miRNA-126, and Let7a-5p using reverse transcriptase polymerase chain reaction methodology and quantitative real-time testing. RESULTS: Analysis showed that miRNA-101, miRNA-21 and miRNA-375 were highly expressed, whereas, miRNA-146-5p, miRNA-126, and miRNA-Let7a-5p showed significantly low levels of expression in T1DM patients compared to controls (p<0.05). In addition, miRNA-101 and miRNA-146 correlated with age at diagnosis of T1DM and disease duration, respectively. Furthermore, multivariate analysis showed that miRNA-126 and Let7a-5p had a significant negative correlation with mean hemoglobin A1c (HbA1c) values. CONCLUSION: Dysregulation of the six miRNAs analyzed suggested a possible role as biomarkers in T1DM. miRNA-101 was correlated with age at diagnosis while miRNA-146 correlated with disease duration. Two further miRNAs correlated with the existing biomarker, HbA1c.
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spelling pubmed-89000822022-03-17 Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM Barseem, Naglaa Fathy Mahasab, Marwa Mohamed Zaed, Ibrahem Fathy Said, Aya Eldesoky A. El Gayed, Eman Masoud Abd J Clin Res Pediatr Endocrinol Original Article OBJECTIVE: Micro RNAs (miRNAs) are gaining acceptance as novel biomarkers for the autoimmune disorders. However, miRNA profiles have not been investigated in individuals at risk of or diagnosed with type 1 diabetes mellitus (T1DM). To study the expression pattern of miRNAs in plasma obtained from patients with T1DM and compare with matched healthy controls. METHODS: Equal numbers of patients with T1DM (90) and healthy-matched control children (90) were assessed for the expression profile of plasma miRNAs including miRNA-101-5p, miRNA-146-5p, miRNA-21-5p, miRNA-375, miRNA-126, and Let7a-5p using reverse transcriptase polymerase chain reaction methodology and quantitative real-time testing. RESULTS: Analysis showed that miRNA-101, miRNA-21 and miRNA-375 were highly expressed, whereas, miRNA-146-5p, miRNA-126, and miRNA-Let7a-5p showed significantly low levels of expression in T1DM patients compared to controls (p<0.05). In addition, miRNA-101 and miRNA-146 correlated with age at diagnosis of T1DM and disease duration, respectively. Furthermore, multivariate analysis showed that miRNA-126 and Let7a-5p had a significant negative correlation with mean hemoglobin A1c (HbA1c) values. CONCLUSION: Dysregulation of the six miRNAs analyzed suggested a possible role as biomarkers in T1DM. miRNA-101 was correlated with age at diagnosis while miRNA-146 correlated with disease duration. Two further miRNAs correlated with the existing biomarker, HbA1c. Galenos Publishing 2022-03 2022-03-03 /pmc/articles/PMC8900082/ /pubmed/34927407 http://dx.doi.org/10.4274/jcrpe.galenos.2021.2021.0080 Text en ©Copyright 2022 by Turkish Pediatric Endocrinology and Diabetes Society | The Journal of Clinical Research in Pediatric Endocrinology published by Galenos Publishing House. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Barseem, Naglaa Fathy
Mahasab, Marwa Mohamed
Zaed, Ibrahem Fathy
Said, Aya Eldesoky A.
El Gayed, Eman Masoud Abd
Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM
title Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM
title_full Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM
title_fullStr Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM
title_full_unstemmed Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM
title_short Genetic Indices Relationship to Hyperglycemia-associated Biomarkers: Consistency with miRNA Expression in Egyptian Children with T1DM
title_sort genetic indices relationship to hyperglycemia-associated biomarkers: consistency with mirna expression in egyptian children with t1dm
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900082/
https://www.ncbi.nlm.nih.gov/pubmed/34927407
http://dx.doi.org/10.4274/jcrpe.galenos.2021.2021.0080
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